108年 - [無官方正解]108 國立高雄大學_研究所碩士班招生考試試題_生命科學系：分子生物學#136720

1. Asymmetrical spacing of the backbones of the DNA double helix generates major and minor grooves.(A)O(B)X

2. A gene can accept occasional changes, which allows organisms to evolve.(A)O(B)X

3. The noncoding strand of a gene is the same as the antisense strand.(A)O(B)X

4. HindII is so named because it was isolated from Haemophilus influenzae bacterium.(A)O(B)X

5. A fragment restricted with EcoRI enzyme can be used for ligation into a plasmid that was restricted with BamHI because both the insert and the plasmid contain sticky ends.(A)O(B)X

6. At high temperature, two complementary DNA strands will anneal.(A)O(B)X

7. RNA polymerase prevents the binding of TFIIF to the promoter region.(A)O(B)X

8. DNA gyrase adds positive supercoils to a negatively coiled DNA molecule.(A)O(B)X

9. Positive supercoliling causes the DNA to form a more compact structure.(A)O(B)X

10. Insertion sequences are transposable elements that can inactivate gene.(A)O(B)X

11. 5'-ACCGTAGGATGCCA-3' is an example of an inverted repeat sequence.(A)O(B)X

12. Antibiotic resistance in bacteria can be transferred using insertion sequences.(A)O(B)X

13. Rearrangement of mammalian cell immunoglobulin genes is responsible for the large diversity of antibodies produced by B-cell.(A)O(B)X

14. Eukaryotic ribosomal RNA gene are transcribed by RNA polymerase II.(A)O(B)X

15. TFIID collaborates with TFIIA to bind to the TATA box.(A)O(B)X

1.Which of the following enzyme is used to synthesize DNA using an mRNA template (A). Alkaline phosphatase (B). Nuclease (C). Reverse transcriptase (D). Taq DNA polymerase

2. Which of the following is a thermostable DNA polymerase (A). Pfu DNA polymerase (B). Taq DNA polymerase (C). Vent DNA polymerase (D). All of the above

3.Minisatellites are (A). Short non-coding repetitive sequences present throughout the chromosome. (B). Short coding repetitive regions on the eukaryotic genome. (C). Are regions of chromosomes after seconday constriction. (D). 10—50 bp sized short sequences within the genes.

4.Nick translation is done by (A). DNA polymerase I (B). DNA polymerase II (C). DNA ligase (D). Kinase

5.The first transgenic plant to be produced (A). Rice (B). Tobacco (C). Maize (D). Cotton

6. The first crop plant genome sequenced (A). Barley (B). Maize (C). Rice (D). Wheat

7.Kary Mullis is associated with (A). Chain termination reaction (B). PCR (C). RAPD (D). RFLP

8. Which of the following was demonstrated by Frederick and Griffiths?(A). Nuclein was the possible genetic material (B). A genetic trait can be transferred from one organism to another (C). Corssing over occurs between chromosomes (D). Protein is a major component of nuclein

9. Who first conducted the X-ray diffraction studies of DNA?(A). Wason and Crick (B). Meselson and Stahl (C). Miescher and McClintock (D). Franklin and Wilkins

10. All of the following are found in RNA except (A). Adenine (B). Deoxyribose (C). Guanine (D). Uracil

11. Rapid cooling of DNA following heating at high temperature will cause (A). The strands to break (B). Rapid renaturation of the strands (C). The strands to remain separated (D). The double helix to fomr rapidly

12. Select the component that is most critical to the function of as plasmid? (A). Tetracycline resistance (B). Ampicillin resistance (C). EcoRI site (D). Origin of replication

13. Which of the following vectors is the best choice for the expression of eukaryotic proteins? (A). M13 (B). Baculovirus (C). Phagemid (D). PUC18

14. Which of the following techniques is not used to quantify the level of gene expression? (A). Southern blotting (B). Northern blotting (C). S1 mapping (D). RNase protection

15. "Snurps" or snRNPs are composed of (A). DNA and RNA (B). RNA (C). DNA (D). RNA and protein

三. 問答題
1. 請說明原核生物 (如大腸桿菌)染色體的複製過程。

2. 請說明真核細胞中 RNA polymerase II 是如何進行轉錄調控，列出所參與的蛋白分子及其功能。