所屬科目：研究所、轉學考(插大)◆分子生物學
1On a polypeptide, which amino acid cannot be phosphorylated?(A) serine (B) valine(C) threonine (D) tyrosine
2Amino acids are the building blocks of:(A) DNAS (B) RNAS(C) Proteins (D) Polysaccharides
3. Which of the following proteins can recognize the Ori sequence in E. coli? (A) DnaA (B) DnaB (C) DnaC(D) DnaG
4Which of the following is a true statement regarding DNA polymerase?(A) It is a processive enzyme(B) It contains proofreading activity(C) It requires a short primer or oligonucleotide to start synthesizing new DNA strands(D) All of the above
5Which activity of DNA polymerase I is also called "proofreading" activity?(A) 5' to 3' polymerase activity (B) 3' to 5' polymerase activity(C) 5' to 3' exonuclease activity (D) 3' to 5' exonuclease activity
6Which of the following is not a "stop codon" ?(A) UAA (B) UGG(C) UGA (D) UAG
7. Which statement is correct? (A) Histone proteins are mainly positively charged. (B) The core histones include H1, H2A, H2B, H3, and H4. (C) Histone proteins interact mainly with the bases of the DNA. (D) None of the above.
8In eukaryotes, DNA replication occurs during:(A) G1 phase (B) G2 phase (C) M phase (D) S phase
9Which of the following molecules is involved in DNA mismatch repair?(A) RecBCD (B) MutH (C) UvrC (D) Spo11
10. Which of the following molecules is involved in DNA nucleotide excision repair?(A) RecBCD (B) MutH (C) UvrC (D) Spo11
11. Which description is false?(A) X-rays are hazardous because they cause DNA double-strand breaks, which are hard to repair.(B) an A to.G mutation is called a point mutation.(C) Human cells are used in the Ames test to determine the carcinogenic effects of chemicals.(D) Non-homologous end joining usually results in DNA mutations.
12. Which transposable element does not use an RNA intermediate to insert into new sites in the host cell genome? (A) DNA transposons (C) Retroviruses (B) Viral-like retrotransposons (D) Poly-A retrotransposons
13. If a species contains 20% adenine ( A ) in its DNA, what percentage of guanine (G) would it also have? (A) 20% (B) 30% (C) 15% (D) 40%
14. The aromatic rings of the bases found in DNA and RNA absorb ultraviolet light with an absorption maximum at: (A) 240 nm (B) 260 nm (C) 280 nm (D) 300 nm
15. DnaG is a:(A) Helicase (B) Topoisomerase (C) Primase (D) DNA ligase.
16. What subunits that are common in all three kinds of the RNA polymerase in eukaryote?(A) Rpb1/Rpb2 (B) Rpb3/Rpb9 (C) Rpb4/Rpb7 (D) Rpb5/Rpb6/Rpb8
17. The sensitivity to a-amanitin for three kinds of RNA polymerase (Pol I, Pol II and Pol III) is:(A) Pol I ＞ Pol II ＞ Pol III (B) Pol I ＞ Pol III ＞ Pol I(C) Pol II ＞ Pol III ＞ PolI (D) Pol III ＞ Pol II ＞ Pol I
18. microRNA(miRNA) is transcribed by:(A) RNA polymerase I(C) RNA polymerase III(B) RNA polymerase II(D) Reverse transcriptase
19. Following the previous question, the 5S rRNA(ribosomal RNA) is transcribed by?(A) RNA polymerase I (B) RNA polymerase II(C) RNA polymerase III (D) RNA dependent RNA polymerase
20. Which one is NOT classified as the transactivation domain of a transcriptional factor?(A) Zinc finger domain (B) Acidic domain(C) Glutamine-rich domain (D) Proline-rich domain
21. Which proteins contains the activity of proofreading during the elongation step in eukaryote transcription? (A) TFIID (B) TFIIE (C) TFIIН (D) TFIIS
22. The triphosphate linkage in the cap structure of a mRNA from 5' to 3' direction is:(A) aBY (B) YBα (C) aßa (D) Y B r
23. The shape of the intron released after trans-splicing is?(A) linear (B) lariat (C) circular (D) Y-shapе
24. In precursor mRNA splicing, which two snRNAs can pair with U6 snRNA?(A) U1 and U2 (B) U2 and U4 (C) U2 and U5 (D) U4 and U5
25. In the infection of E. coli by λ phage, which description for the binding ability of cro is correct?(A) OR1 ＞ OR2 ＞ OR3 (B) OL1 ＜ OL2 ＜ OL3(C) OR1 ＞OR2 =OR3 (D) OL1 ＜ OL2 = OL3
26. The ribosome-binding site in a mRNA is named as?(A) Shine-Dalgarno sequence (B) Kozak sequence(C) Okazaki sequence (D) Upstream Activating Sequence (UAS)
27. Primary miRNA can be digested to precursor miRNA by?(A) Argonaute (B) Slicer (C) DGCR8/ Pasha (D) Dicer
28. The first amino acid that incorporated into the P site of ribosome during the protein synthesis inprokaryote is:(A) N-formyl Methionine (B) Methionine(C) either N-formyl Methionine or Methionine (D) Serine or Threonine
29. The property of peptidyl transferase is confirmed as?(A) RNA (B) both RNA and protein (C) protein (D) not identified yet
30. Which method is NOT used for detecting protein-protein interaction directly or indirectly:(A) Yeast two-hybrid assay (B) Western blot(C) Co-immunoprecipitation (D) Pull-down assay二、簡答題:(10題,每題2分,共計20分)
31. Name the two most common secondary structures of proteins.
32. What enzyme can use RNA as a template to synthesize DNA?
33. What enzyme removes excessive supercoiling ahead of the replication fork?
34. Which subunit increases the processivity of DNA polymerase III?
35. Name two kinds of histone modifications.
36. The ways for transcriptional termination in prokaryote.
37. The small RNAs that are required for mRNA splicing and rRNA processing, respectively.
38. The RNA component that is responsible for the 5' end processing of tRNA.
39. Explain the Kozak's rule for translation and its biological function.
40. List at least two functions for the cap structure of the mRNA.
41. Please illustrate and describe the "base excision repair" mechanism in E.coli.
42. Please describe the molecular process (including all the molecules involved) of homologousrecombination in E. coli.
43. Please explain the Wobble concept.
44. Please describe the positive and negative regulation of lac operon.
45. Please at least list two enzymes that are involved in RNA editing.
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