112年 - 112 台灣聯合系統大學碩士班招生考試：生物化學#117374

1. Which of the following respiratory complexes contains only the nuclear DNA-encoded proteins? (A) Complex I(B) Complex II (C) Complex II (D) Complex IV (E) Complex V.

2. a helix of a polypeptide has a pitch of.___nm/turn. (A) 0.34 (B) 0.54 (C) 3.4 (D) 3.6 (E) 5.4.

3. Which of the following descriptions about Hb (hemoglobin) is incorrect? (A) One Hb can bind 4 O₂ molecules (B) HbA has an α₂β₂ structure (C) HbF has an α₂γ₂ structure (D) HbF has a lower affinity for 2,3-BPG than does HbA (E) Decreased 2,3-BPG levels are found in the blood of smokers.

4.Which of the following descriptions about the Michaelis-Menten rate equation is incorrect? (A) Km measures the substrate concentration at which the reaction rate is V_max/2 (B) kat is the turnover number that measures the catalytic process (C) The ratio of Kcat/Km is a convenient measure of enzyme efficiency (D)k_cat=V_max/K_M (E) A competitive inhibitor increases the apparent KM.

5. To visualize a DNA band,_______ is commonly used after agarose gel electrophoresis. (A) Uridine (B) EBr (C) coomassie brilliant blue (D) CNBr (E) BUdR.

6.A DNA segment of 100 base pairs in the Z form. What is its approximate molecular weight? (A)33(B)65(C)330 (D)650 (E) 3,300 KD.

7. Absorbance at______ nm is frequently used to determine the concentration of DNA. (A) 200 (B) 260 (C) 280 (D)400(E) 540.

8. Which of the following compounds has the lowest molecular weight? (A) guanine (B) guanosine (C) guanylate (D) cytosine(E) cytidine.

9.Which of the following descriptions regarding disaccharide is incorrect? (A) Sucrose is a non-reducing sugar (B) Sucrose is a-D-glucopyranosy! (1→4) β-D-fructofuranoside (C) Maltose has an a(1→4) linkage (D) Cellobiose is B-D-glucopyranosyl (1→4) β-D-glucopyranose (E) Lactose is a reducing sugar.

10.Which of the following restriction endonucleases is an isoschizomer of Xbal (TCTAGA)?(A) EcoRI ( (B) Spel (ACTAGT) (C) BamHI (GGATCC) (D) SalI (GTCGAC)(E) EagI (CGGCCG).

11.ch of the following proteins is involved in scrapie? (A) collagen (B) elastin (C) kerati (D)prion (E)chaperonine.

12. NP-40 is a nonionic surfactant that denatures proteins by disrupting. _______? (A) hydrogen bonds (B) disullide bridges (C) hydrophobic interactions (D) salt bridges (E) covalent bonds.

13. How many stereoisomers for an aldohexose? (A) 2 (B) 4 (C) 8 (D) 16 (E) 32.

14. The chemical bond between ribose and base of a nucleotide is a(n) _______ bond. (A) ether (B) ester (C) glycosidic (D) aldehyde(E) ketone.

15. Which of the following lipids are especially common in the membranes of brain and nerve cells? (A) cerophospholipids (B) waxes (C) fatty acids (D) glycosphingolipids (E) glycogl

16. In humans, mitochondrial DNA encodes_______ proteins. (A) 37 (B) 22 (C) 13 (D) 2 (E) 0.

17. The catalytic triad of serine proteases consists of____,____ ,and____ ? (A) Ser, His, and Asp (B) Phe, Trp, and Tyr (C) Asp, Glu, and Pro (D) His, Asp, and Glu (E) Ser, Thr, and Cys.

18.Which scientist proposed the model of chemiosmotic coupling? (A) P. Mitcheli (B) J. D. Watson and H. C. Crick (C) A. D. Hershey and M. Chase (D) S. B. Prusiner (E) F. Sanger

19.Which of the following amino acids contains only one codon? (A) Ala (B) Lys (C) Tyr (D) Trp (E) Arg.

20. B form DNA has______residues per turn. (A) 1 (B) 2 (C) 3 (D) 5 (E) 10

21. The Southern blotting method is normally used for detection of (A) proteins (B) DNA (C) RNA (D) lipids (E) carbohydrates.

22. Which of the following tautomeric forms of fructose exists in sucrose? (A) α-pyranose (B)β-pyranose (C)α- furanose (D) β-furanose (E) all of the above.

23.Oselta mivir (tamiflu) is effective in treating HIN1 influenza infection, because it has a structun similar to(A)hyaluronic acid (B) heparin (C) N-acetylgalactosamine (D) N-acetylglucosamine (E) sialic acid.

24. Which of the following compounds is frequently used by emergency medical personnel as an antidote for cyanide poisoning? (A) CO (B) rotenone (C) antimycin A (D) azide (E) NaNO₂

25. h pair of enzymes listed below generates NADPH? (A) glucose-6-phosphate dehydro phogluconate dehydrogenase (B) malic enzyme and glucose-6-phosphate dehydrogenase (C) citr and malic enzyme (D) 6-phosphogluconate dehydrogenase and fructose-bisphosphatase-1 (E) fructose- bisphosphatase-1 and hexose kinase.

26. How many CO₂ molecules are released for each acetyl-CoA entering the glyoxylate cycle in plant cells? (A) O (B)1(C)2(D)3(E) 4.

27. most important regulatory step in glycolysis is catalyzed by_____. (A) hexokinase (B) pyru glyceraldehyde-3-phosphate dehydrogenase (D) phosphofructokinase (E) phosphoglycerate kinase.

28.Which of the following enzymes is not involved in the glyoxylate cycle? (A) aconitase (B) isocitrate lyase (C)malate synthase (D) malate dehydrogenase (E) isocitrate dehydrogenase.

29. In a Lineweaver-Burk double reciprocal plot, the intercept of the x-axis equals______.(A) K_M (B) -1/K_M (C)V_max (D) 1/V_max (E) k_cat/K_M.

30. phofructokinase-1 (A) is activated by ADP and citrate (B) is activated by ADP and hosphate (C) is activated by citrate and fructose-2,6-bisphosphate (D) is activated by ATP 2,6-bisphosphate (E) is inhibited by ATP and fructose-2,6-bisphosphate

31. Which amino acid lacks a chiral a-carbon? (A) Gly (B) Trp (C) Tyr (D) His (E) Pro.

32. Amino acids can be covalently linked together by formation of a(n)_______. bond. (A) ether (B) ester (C) peptide (D) glycosidic (E) none of the above.

33. many moles of FADH₂ mnolecules are produced in the TCA cycle per molecule of acetyI-CoA oxidized(A) 5(B) 4(C)3(D)2(E) 1.

34.The enzymes that catalyze the hydroxylations of proline and lysine residuesin collagen requires______.(A) vitamin A (B) vitamin C (C) vitamin E (D) vitamin B (E) selenocysteine and pyrrolysine.