112年 - 112 國立成功大學_碩士班招生考試_生物化學暨分子生物學研究所：生物化學#118677

科目：研究所、轉學考(插大)◆生物化學 | 年份：112年 | 選擇題數：0 | 申論題數：16

試卷資訊

所屬科目：研究所、轉學考(插大)◆生物化學

選擇題 (0)

申論題 (16)

1. Name five amino acid residues that can form hydrogen bonds with others. (5 pts)

a) Draw or describe the shape of X-binding curve of protein A. (2 pts)

b) Describe the effect of adding compound Y. (3 pts)

3. How competitive inhibitor affects enzyme catalysis? (2 pts) Why transient state analogues are generally more ideal competitive inhibitors than substrate analogues? (3 pts)

4. Please describe how Sanger sequencing works in sufficient detail. (3 pts)

(A) If you are the technician, how do you remove the contaminated cells and establish the new patient's myeloma cell line? (5 pts)

(B)If the neww myeloma cell line is successfully established, what are the potential appli biomedicine? (please describe in detail) (7 pts)

6. Please describe in sufficient detail of how tetrahydrofolate (THF) and its derivatives contribute the associated carbon units to biosynthesis. (10 pts)

a). The presence of glycine in o helices. (3 pts)

b). The presence of proline in ou helices. (3 pts)

C)Tyrosine and tryptophan respectively at residue position i and it4 of a α helix. (4pts)

8. Please describe the "reciprocal regulation" of glycolysis and gluconeogenesis by the hormone, glucagon, ough the modulation of fructose-2,6-bisphosphate concentration in cytosol.(10%)

9. Please describe the regulation of glycogenolysis and glycogenesis by the hormone, insulin, through modulation of the phosphorylation state of glycogen phosphorylase and glycogen synthase by protein phosphatase 1 (PP1).(10%)

10. Please name the enzymes that are involyed in the production of NADH in the citric acid cycle, and how these enzymes are regulated. (10%)

11. Uncoupling compounds, such as carbonylc yanide-p-trifluoromethoxyphenylhydrazone (FCCP), can disrupt the proton-motive force across the inner membrane of mitochondria. Please desctibe in detail mode of action of FCCP, and the consequence of cells treated by FCCP(10%)

12. Dietary deficiency of niacin (vitamin B3) causes Pellagra in humans. Please describe why niacin and its derivatives are so important in the general oxidation and reduction reactions in metabolism? (10%)