所屬科目:研究所、轉學考(插大)◆生物化學
13. Which of the following enzymes converts stearoyl-CoA to oleoyl-CoA? (A) △ 5 desaturase. (B) △ 6 desaturase. (C) △ 7 desaturase. (D) △8 desaturase. (E)△9 desaturase.
45. An analysis of chromosomal DNA, using the Southern blot technique, involves the following five major steps: ①Autoradiography; ②Blotting; ③ Cleavage;④Electrophoresis; ⑤Hybridization;Which of the following sequences of steps best illustrates this technique? (A)①②③④⑤ (B) ①③②④⑤ (C) ③⑤②④①(D) ③②⑤④①(E) ③④②⑤①
48. A biochemist is attempting to separate a protein X from protein C in a solution. The proteins have the following properties: What type of protein separation techniques might be the best one to separate protein X from protein C? (A) Gel filtration (B) SDS-polyacrylamide gel electrophoresis (C) Affinity chromatography (D) Ion-exchanger chromatography (E) Agarose gel electrophoresis
49. A sequence of amino acids in a certain protein is found to be –Ser–Gly–Pro–Gly–. The sequence is most probably part of a(n)______. (A) β-turn (B) parallel β-sheet (C) α-helix (D) α-sheet (E) antiparallel β-sheet
50. Which of the following is (are) true of the oxidation of 1 mol of palmitate (a 16-carbon saturated fatty acid; 16:0) by the β-oxidation pathway, beginning with the free fatty acid in the cytoplasm?①Activation of the free fatty acid requires the equivalent of two ATPs.② Inorganic pyrophosphate (PPi) is produced.③Carnitine functions as an electron acceptor.④8 mol of FADH2 are formed.⑤8 mol of acetyl-CoA are formed.⑥There is no direct involvement of NAD+ . (A)①and⑤ (B) ①②and⑤(C) ①② and⑥ (D) ①③ and ⑤ (E)④ and⑤