試題詳解

15. Shown below is the plasmid backbone for small hairpin RNA (shRNA) used in the National RNAi Core

(A) The plasmid carries a gene for selection of E. coli that carry it
(B) The plasmid carries features that facilitate its integration into mammalian genome
(C) The plasmid carries a gene for selection of mammalian cells that carry it
(D) shRNA can be inserted to the plasmid and can be expressed driven by the U6 promoter
(E) The plasmid itself is infectious because it contains HIV elements