複選題

17.About transcription, which is (are) CORRECT?
(A) A transcriptional factor can bind to an enhancer which is far away from the promoter.
(B) The TATA box is located at all promoters of RNA Pol II transcribed gene.
(C) The TATA box is usually bound by a TATA box binding protein
(D) Histone methylation opens chromatin
(E) Histone acetylation opens chromatin

複選題13. Regarding to cancer, which is (are) CORRECT? (A) PD1 expression level is critical for the success of the PD1 antibody-conducted immunotherapy. (B) RB is a CDK inhibitor at G1 (C) RB binds the G1cyclin D (D) HIF1a stabilization is critical for metastasis (E) Telomerase expression is required for its maintenance

複選題14. About mismatch repair, which is (are) CORRECT? (A) MutS binds to the methylation site (B) UvrB bends the DNA (C) UvrC cleavages the sites around the damage (D) Only one strand is repaired (E) The pathway is frequently damaged in liver cancer

複選題15. Which methods can be used to change the expression of a wild-type gene? (A) Small interference RNA (B) Antisense RNA (C) MicroRNA (D) TALEN gene editing (E) CRISPR/Cas9 editing

複選題16.About chromatin, which is (are) CORRECT? (A) Genes at heterochromatin is usually silent (B) Telomeric genes are highly expressed (C) Histone tails are usually highly methylated (D) H3K4 is an active mark (E) X chromosome is inactivated by a long non-coding RNA

18. Which of the following factors is essential for mRNA maturation in eukaryotes? (A) TATA-box binding protein (B) Ribosome (C) 5'-capping complex (D) Splicesome (E) RNA pol II polymerase

19. Which of the following sequence has the highest melting temperature? (A) Ggattc cctagg (B) Ggaccc cctagg (C) Ggaaac cctagg (D) Ggattc tttagg (E) Ggattc aatagg

20. Which of the following pathways need helicase? (A) DNA replication (B) Homologous recombination (C) Gene transcription (D) RNA splicing (E) RNA editing

21. Which of following statements for nucleic acid is (are) TRUE: (A) C, T are Purines (B) There are three hydrogen bonds between CG pairing (C) During the process of free nucleotides becoming RNA, every single nucleotide added, two phosphate groups are released. (D) The higher the GC content, the lower the Tm value is. (E) RNA at neutral pH buffer (pH=7) has no charge

22. What the followings are True when one design a set of primer for cloning: (A) Introducing extra sequences that would give us a restriction site in primer can be added at either 5' or 3' end of primer (B) Annealing temperature should only consider the sequence that could based paired with the target gene (C) Only if a N-terminus protein tag has been introduced into vector backbone, one should consider whether introduced restriction sites in the primer result in frame shift in the final protein products (D) Length of primer can be as short as 5 nucleotides (E) As long as melting temperature reaches to the target temperature, nucleotide composition is not affect the primer efficiency

複選題23. Which of the following statements for mRNA is (are) TRUE: (A) Prior to 5' mRNA capping, there are three phosphate at 5' end of mRNA (B) Cap structure is dispensable for protein translation initiation in eukaryotic mRNA. (C) Poly（Ａ） tail of the mRNA is encoded in the DNA sequence (D) One eukaryotic mRNA corresponding to one gene whereas one prokaryotic mRNA containing multiple gens. (E) RNA splicing is one of the first discovered enzymatic reaction that can be catalyzed by Ribozyme.