試題詳解

27. Which of the following is correct regarding yeast three-hybrid analysis?
(A) A useful tool in analyzing RNA-protein interactions
(B) It contain a fusion protein of LexA, which binds the reporter construct, and MS2 which specifically binds an RNA tag that has been added to every RNA produced by the plasmid library.
(C) It needs a plasmid library that contains random sequences from the genome tagged with a unique hairpin sequence that MS2 will bind.
(D) It requires a reporter construct that contains a LexA operator (binding site) and a reporter gene.
(E) It needs a fusion protein of the protein of interest and two tags - the calmodulin binding site and protein A.