10. Which of the following templates is NOT a substrate for polymerase chain reaction (PCR)?
(A)Double strand DNA
(B)Single strand DNA
(C)Circular form of DNA
(D)mRNA
(E)Plasmid DNA.

11. At replication forks in E. coli: (A)DNA helicases make endonucleolytic cuts in DNA. (B)DNA primers are degraded by exonucleases. (C)DNA topoisomerases make endonucleolytic cuts in DNA. (D)RNA primers are removed by primase. (E)RNA primers are synthesized by primase.

12. The function of the eukaryotic DNA replication factor PCNA (proliferating cell nuclear antigen) is similar to that of the B-subunit of bacterial DNA polymerase III in that it: (A)facilitates replication of telomeres. (B)increase the processivity of replication. (C)has a 3' → 5' proofreading activity. (D)increases the speed but not the processivity of the replication complex. (E)participates in DNA repair.

13. Which of the following statements regarding plasmid cloning vectors is correct? (A)Circular plasmids do not require an origin of replication to be propagated in E. coli. (B)Foreign DNA fragments up to 45,000 base pairs can be cloned in a typical plasmid. (C)Plasmids do not need to contain genes that confer resistance to antibiotics. (D)Plasmid vectors must carry promoters for inserted gene fragments. (E)The copy number of plasmids may vary from a few to several hundred.

14. In homologous recombination in E. coli, the protein that assembles into long, helical filaments that coat a region of DNA is: (A)RecA protein. (B)RecBCD enzyme. (C)DNA methylase. (D)DNA polymerase. (E)Histone.

15. Apolipoprotein AI (Apo-AI) deficiency is associated with (A)low plasma total cholesterol (B)high plasma total cholesterol (C)high plasma triacylglycerol (D)low plasma LDL-cholesterol (E)low plasma HDL-cholesterol

16. Which of the following is not required for synthesis of arachidonic acid (C20:4, n-6) from linoleic acid (C18:2, n-6)? (A)Δ4-desaturase (B)Δ5-desaturase (C)Δ6-desaturase (D)Elongase (E)Malonyl-CoA

17. Which enzyme is the key enzyme for bile acid biosynthesis? (A)Acetyl-CoA carboxylase (B)7α-hydroxylase (C)HMG-CoA reductase (D)HMG-CoA lyase (E)Acyl-CoA synthetase

18. Which of the following is responsible for the generation of LDL from VLDL? (A)HMG-CoA reductase (B)HMG-CoA lyase (C)Hormone sensitive lipase (D)Lipoprotein lipase (E)Pancreatic lipase

19. What molecule is it that provides 8 electrons for nitrogen fixation? (A)Glucose (B)Oxaloacetate (C)Pyruvate (D)Acetyl-coA (E)ATP

20. What enzyme is it that mediates nitrogen entry into higher organisms? (A)Glutamine synthetase (B)Glutamate-pyruvate transaminase (C)Carbamoyl phosphate synthetase I (D)Arginase (E)N-acetylglutamate synthase

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