12. elF4F is a complex of eukaryotic translation initiation factors that binds to the cap structure of mRNA. Which of the followings is one of the components of elF4F?
(A)elF4A
(B) elF2B
(C) elf1
(D) elF3
(E) elF5

13. There are several kinds of RNA polymerases in eukaryotic cells. What is the RNA polymerase that makes tRNA in eukaryotic cells? (A) RNA polymerase I (B) RNA polymerase I| (C) RNA polymerase III (D) RNA polymerase IV (E) RNA polymerase V

14. Which of the followings is not a posttranscriptional RNA processing event in eukaryotic cells? (A) Phosphorylation of translational initiation factor (B) RNA splicing (C) RNA editing (D) mRNA capping (E) polyadenylation of mRNA

15. Which of the followings is one of the mechanisms that repair DNA damage in eukaryotic cells? (A) Phosphorylation repair (B) Double-strand break repair (C) Ubiquitination repair (D) Sumoylation repair (E) tRNA-mediated repair

複選題16. Which of the following statement about isoenzymes and isoforms of enzymes is correct? (A) Different isoenzymes have the same catalytic properties for the same reaction. (B) Both isoenzymes and isoforms can have tissue-specific distribution. (C) Phosphorylation is one mechanism leading to the formation of different isoenzymes. (D) Different isoforms are encoded by different genes. (E) Different isoenzymes are encoded by the same genes.

17. Which of the following statement is correct about reversible inhibition of enzymatic reactions? (A) Km is reduced in noncompetitive inhibition. (B) A competitive inhibitor is structurally different from the substrate of the enzyme. (C) Vmax in competitive inhibition is unaffected. (D) When plotting as Lineweaver-Burk Plot, the straight line for the condition with uncompetitive inhibitor and that of the condition without an inhibitor have the same Y-axis intercept. (E) None of about is correct.

18. Which of the following statement about coenzymes and prosthetic groups is not correct? (A) coenzyme is an organic non-protein compound that binds with an enzyme to catalyze a reaction. (B) Coenzymes are bound only momentarily to the enzyme during the course of the reaction. (C) Prosthetic group binds to the enzyme to form the active holoenzyme. (D) Pyridoxal-5'-phosphate is the prosthetic group of dehydrogenases. (E) NAD*/NADH and NADP*/NADP are classified as coenzymes of the enzymes.

19. Which enzyme catalyzes the reaction NDP → dNDP? (A) thymidylate synthase (B) ribonucleotide kinase (C) hypoxanthine phophoribosyI transferase (D) topoisomerase (E) ribonucleotide reductase

20. Double stranded DNA molecules can be cleaved at specific recognition sites by (A) RNA polymerase.(B) DNA ligase. (C) DNA polymerase. (D) gyrase. (E) restriction endonucleases.

21. Which one of the following is the major component of cell membrane? (A) cholesterol (B) triacylglycerol (C) terpene (D) phospholipid (E) sphingolipid

22. GLUT 1 is a transporter for glucose uptake. Which mechanism is used by this receptor? (A) simple diffusion (B) facilitated transportation (C) activate transportation (D) act as an ionophore (E) none of above

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