15. During purification of an antibody with a protein G affinity column, 0.1 M acetic acid was used as the elution buffer. After elution, which of the following buffers (equal volume) is most suitable for bringing the pH value of the eluate to ~8.0? (Tris has a pKa of 8.1)
(A) 0.2 M Tris base
(B) 0.2 M Tris-HCl
(C) 0.2 M Tris, pH 8.0
(D) 0.1 M NaOH

1. If we have one DNA sequence with a 5-minutes PCR cycle time. After 1 hour, how many DNA sequence can we get? (A) 1024 (B) 2048 (C) 3072 (D) 4096

2. Which statements of SDS PAGE is correct? (A) SDS acts as a detergent and attaches to the protein, so the protein has positive charge. (B) Direction of protein movement is from positive charge (+) to negative charge (-). (C) APS (ammonium persulfate) as a buffer of SDS PAGE.(D) High molecular weight protein would stay in the upper section of the SDS PAGE.

3.   A   is a common experimental method to detect protein in a biological sample.   B   can investigate DNA in the biological sample. Dr. Kary Banks Mullis builts a method   C   which can ampilify DNA in a short period of time. Based on these description, what are methods   A  ,   B   and   C  ? (A) A: Southern blot, B: Eastern blot, C: CPR. (B) A: Southern blot, B: Western blot, C: CPR. (C) A: Western blot, B: Northern blot, C: PCR. (D) A: Western blot, B: Southern blot, C: PCR.

4. Which statement is correct? (A) Nucleoside contains nitrogen base, pentose sugar and phosphate group (B) Watson and Crick discovered RNA is a double helix structure (C) Gout patients should decrease ingestion of high purine food to release symptom (D) DNA is composed of A, T, G, C. A is a kind of pyrimidine.

5. 2D-PAGE is a method to investigate protein. Differential migration of analytes in 2D-PAGE let us measure protein expression difference between two samples. The rate of migration does not depend on? (A) Isoelectric point (B) Fluorescence (C) Protein size (D) Molecular weight

6. Which two amino acids can bind together and form disulfide bond? (A) Methionine and cysteine. (B) Serine and threonine. (C) Lysine and tyrosine. (D) Cysteine and cysteine.

7. Which one is the lowest molecular weight carbohydrate? (A) Cellulose (B) Glyceraldehyde (C) Glucose (D) Xylose

8. The effect of the deletion of one copy of the gene encoding p53 is different from the effects caused by other p53 mutations. For example, some loss-of-function mutations in the DNA-binding domain of p53 cripple its function as a transcription regulator. Such a mutation in only one copy of the p53 gene can be enough to confer a p53 loss-of-function phenotype, even when the other copy of the gene on the homologous chromosome is wild type. This is because ... (A) p53 is a proto-oncogene. (B) these mutations are recessive. (C) p53 is a tumor suppressor. (D) p53 forms a tetramer.

9. Mutations in two important cancer-critical genes, encoding p53 and Rb, respectively, are commonly found in cancers. What type of mutations are these expected to be? (A) Loss-of-function mutations in both genes (B) Loss-of-function mutation in p53 and gain-of-function mutation in Rb (C) Gain-of-function mutation in p53 and loss-of-function mutation in Rb

10. Which enzyme of the inosinic acid biosynthesis pathway is the major control point for purine synthesis? (A) phosphoribosyl pyrophosphate amidotransferase (B) glycinamide ribonucleotide transformylase (C) formylglycinamidine ribonucleotide amidotransferase (D) 5-aminoimidazole ribonucleotide synthetase

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