試題詳解

15. During purification of an antibody with a protein G affinity column, 0.1 M acetic acid was used as the elution buffer. After elution, which of the following buffers (equal volume) is most suitable for bringing the pH value of the eluate to ~8.0? (Tris has a pKa of 8.1)
(A) 0.2 M Tris base
(B) 0.2 M Tris-HCl
(C) 0.2 M Tris, pH 8.0
(D) 0.1 M NaOH