16. In the nomenclature of restriction enzymes, like BamHI, please answer what " B " means.
(A)Genus
(B) Species
(C) Strain
(D) The order of discovery
(E) Isoform

17.Which of the following statement for the lambda phage switching between lytic and lysogenic pathways is NOT correct. (A)As long as the cI gene is expressed, lambda phage keeps in the prophage state (B) cl repressor binds to the OR1 to inhibit the expression of cro gene (C) cI repressor cleaves itself and can not form dimer, which is required for the repressor activity (D) After UV irradiation, RecA binds to ssDNA and cleaves cI repressor, then lambda phage is switched to lytic pathway (E) RecA possesses co-protease activity

18. Which of the following statement for the regulation of eukaryotic gene transcription is NOT correct. (A)The promoter region, enhancer and repressor are the Cis-elements for transcription (B) Histone modifications (Epigenetics) involve in the chromatin structure changes and gene regulation (C) Methylation on deoxycytidine of CpG island causes the inactivation of gene expression (D) Antibodies are generated through the loss of genomic information (E) One transcription factor can regulate multiple genes

19. Which of the following statement for the control of the regulatory transcription factors is NOT correct. (A)Specific synthesis of transcription factors (B)Covalent modifications of protein (such as isoprenylation) (C)Receptor and ligand binding (such as glucocorticoid receptor and its ligand) (D)Availability of functional factor in nucleus (E) Dimerization with active partner (such as Jun and Fos)

20. Which of the following technique can NOT be used for mRNA quantitation. (A)Southern blot analysis (B)Reverse transcription-quantitative polymerase chain reaction (real-time PCR) (C)cDNA microarray (D)Direct RNA sequencing (E) Next generation sequencing (NGS)

21. Which of the following post-translational modification is common for protein to target to the plasma membrane? (A)Acetylation (B)Phosphoryiation (C)Ubiquitination (D)Farnesylation (E)Methylation

22. How many membrane-spanning domains do G-protein coupled receptors possess? (A)1 (B)3 (C)5 (D)7 (E)9

23. Which of the following receptor family mediates bi-directional (both inside-out and outside-in) signaling? (A)G-protein coupled receptors (B) Gated ion channels (C) Integrins (D) Nuclear Receptors (E) Receptor Tyrosine Kinase

24. Which of the following hormone has the longest half-life in serum? (A)Thyroxine (T4) (B) Thromboxane A2 (C) Thyrotropin-Releasing Hormone (TRH) (D) Thyroid-Stimulating Hormone (TSH) (E) They have the same half-life

25. Which of the following enzymes can generate H2O after the completion of their catalyzed reactions? (A)Glyceraldchyde 3-phosphate dehydrogenase and Glutathione peroxidase (B) Glutamate dehydrogenase and Glutathione reductase (C) Glutamate dehydrogenase and Glutaminase (D) Cytochrome C oxidase and Cytochrome P450 (E) Glucose 6-phosphate dehydrogenase and Galactose epimerase

26. Which of the following reactions can produce succinate as a product? (A)α-ketoglutarate dehydrogenase (B) Succinate dehydrogenase (C) Phenylalanine hydroxylase (D) Tryptophanase (E) Proline hydroxylase

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