16. A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme EcoRI, which cuts the plasmid at a single site in the tetracycline-resistance gene. The plasmid DNA is then annealed with an EcoRI digest of human DNA, ligated, and used to transform E. coli cells. What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid? (A) Ampicillin. (B) Tetracycline. (C) Ampicillin and tetracycline (D) Any antibiotic will do. (E) The agar plate should not contain any antibiotic.

1. Compare the two reaction coordinate diagrams below and select the answer that correctly describes their relationship. In eachcase, the single intermediate is the ES complex.Free energy, (A) Diagram (a) describes a strict "lock and key" model, whereas diagram (b) describes a transition-state complementarity model. (B) The activation energy for the catalyzed reaction is #5 in diagram (a) and is #7 in diagram (b). (C) The activation energy for the uncatalyzed reaction is given by #5 + #6 in diagram (a) and by #7 + #4 in diagram (b). (D) The contribution of binding energy is given by #5 in diagram (a) and by #7 in diagram (b). (E) The ES complex is given by #2 in diagram (a) and #3 in diagram (b).

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