17.During DNA replication, Okazaki fragments are formed on the:
(A) lagging strand
(B) leading strand
(C) template strand
(D) coding strand

18.Which will be modulated by phosphorylation? (A) Serine (B) Threonine (C) Tyrosine (D) All of the above

19.Which following phosphorylation is not coupled by ATP? (A) glucose → glucose-6-phosphate (B) fructose-6-phosphate → fructose-1,6-bisphosphate (C) glyceraldehydes-3-phosphate → 1,3-bisphosphoglycerate (D) phosphoenolpyruvate → pyruvate

20.Which of the following is the precursor for purine biosynthesis? (A) Adenine (B) Hypoxanthine (C) Aminopterin (D) thymidine

21.Deamination of 5-methyl cytosine leads to: (A) C to A transversion mutations (B) C to A transition mutations (C) G to A transversion mutations (D) G to A transition mutations

22.What is the major product for Glyoxylate cycle? (A) Citrate (B) succinate (C) oxaloacetate (D) pyruvate

23.The isoelectric point of alanine is 6.0. If alanine is dissolved in the pH 8.0 buffer and subjected to electrophoresis, it will: (A) not migrate to either anode or cathode (B) migrate to the cathode (negative pole) (C) migrate to the anode (D) some will migrate to the anode and some to the cathode

24.Continuing from the above question, this product is for: (A) TCA cycle in bacteria (B) gluconeogenesis in animal (C) TCA cycle in animal (D) gluconeogenesis in plant

25.The mutant form of prions is the causative agent of Mad Cow Disease in human. The major difference between mutant prions and the normal prions is that: (A) the mutant prions have a large percentage of α-helix whereas the normal prions have more β-sheets (B) the normal prions have a large percentage of α-helix whereas the mutant prions have more β-sheets (C) both the mutant prions and the normal prions have more β-sheets (D) both the mutant prions and the normal prions have a large percentage of α-helix

26. Which of the enzyme has higher substrate affinity? (A) A (B) B (C) C (D) D

27.Gel filtration chromatography separates the molecules based on the: (A) specific binding (B) net charge (C) size exclusion (D) precipitation

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