2. FLAG-tag is often introduced to a protein for Western blotting, immunoprecipitation, or recombinant protein purification. It consists of an amino acid sequence DYKDDDDK. When a FLAG-tag is introduced a recombinant protein, how would it affect the net charge of the recombinant protein when buffered at neutral pH?
(A)+4
(B)+1
(C)-2
(D)-3
(E)-5

98. Look at the map. Which pathway incurs the greatest expense due to toll stations? (A) Pathway 1. (B) Pathway 2. (C) Pathway 3. (D) Pathway 4.

99. Which route is the least recommended for novice drivers? (A) Pathway 1. (B) Pathway 2. (C) Pathway 3. (D) Pathway 4.

100. Which route is best suited for drivers who are short on time? (A) Pathway 1. (B) Pathway 2. (C) Pathway 3. (D) Pathway 4.

1. Post-translational modifications are essential for protein functions. Which of the following post-translational modifications will not occur to lysine? (A) Acetylation (B) Phosphorylation (C) Ubiquitination (D) Methylation (E) Glycation

3. Which of the following amino acids contains a β-branched side chain? (A) Alanine (B) Glutamate (C) Isoleucine (D) Proline(E) Tryptophan

4. Which statement is true regarding solutes that are soluble in water? (A) The solute must be ionic. (B) The water-solute interactions must be more energetically favorable than water-water interactions. (C) The solute must be nonpolar. (D) The undissolved solute particles must only be attracted to each other by van der Waals interactions. (E) The solute must be hydrophilic.

5. The ion channel that opens in response to acetylcholine is an example of a _________signal transduction system.(A) G protein (B) ligand-gated (C) receptor-enzyme (D) serpentine receptor (E) voltage-gated

6.All of the following statements are true about free fatty acids released from adipose tissue expect(A) Fatty acids are hydrophobic.(B) Fatty acids are not soluble in blood, the cytosol, or any other water solution.(C) Fatty acids are transported in the blood by albumin.(D) Fatty acids bind to a hydrophilic binding pocket of albumin.(E) The released fatty acid is taken up by muscle and liver.

7. The synthesis of both glycerophospholipids and triacylglycerols involves (A) serine. (B) phosphatidylethanolamine. (C) phosphatidic acid. (D) phosphocholine. (E) phosphatidic acid phosphatase.

8. In the normal oxidation of an odd chain fatty acid, all of the following would be part of the process except (A) Each odd chain fatty acyl CoA will produce one propionyl-CoA. (B) Propionyl-CoA enters the mitochondria using the carnitine-palmitoy1transferase I system. (C) Propiony1-CoA is converted to methylmalonyl-CoA. (D) Methylmalony1-CoA is converted to succiny1-CoA. (E) The oxidation of an odd chain fatty acid requires biotin to produce propiony1-CoA.