試題詳解

2. FLAG-tag is often introduced to a protein for Western blotting, immunoprecipitation, or recombinant protein purification. It consists of an amino acid sequence DYKDDDDK. When a FLAG-tag is introduced a recombinant protein, how would it affect the net charge of the recombinant protein when buffered at neutral pH?
(A)+4
(B)+1
(C)-2
(D)-3
(E)-5