22. All are important reasons to phosphorylate glucose in the first step of glycolysis EXCEPT: (A) the large positive free energy is important in getting the pathway started (B) glucose-6-phosphate has a negative charge preventing transport out of the cell (C) the concentration of free glucose in the cell is low favoring influx of glucose (D) phosphorylation keeps the glucose in the cell (E) regulatory control can be imposed only at a reaction not at equilibrium