25. You have inserted the gene for human growth factor into the E. coli lactose operon, replacing the structural genes with the gene for human growth factor. What substance must you add to your culture of bacteria to cause them to produce human growth factor for you?
(A) human growth factor
(B) transcription factors
(C) operator protein
(D) allolactose
(E) repressor protein

26. Which of the following is a problem with the long-term use of glucocorticoids, such as cortisone, to treat inflammation? (A) abnormal blood clotting, which leads to excessive bruising (B) low blood glucose levels (C) suppression of the immune system (D) sleeplessness (E) long-term elevated blood pressure

27. Which of the following best describes siRNA? (A) a molecule, known as Dicer, that can degrade other mRNA sequences (B) a portion of rRNA that allows it to bind to several ribosomal proteins in forming large or small subunits (C) a single-stranded RNA that can, where it has internal complementary base pairs, fold into cloverleaf patterns (D) a double-stranded RNA that is formed by cleavage of hairpin loops in a larger precursor (E) a short double-stranded RNA, one of whose strands can complement and inactivate a sequence of mRNA

28. What are single nucleotide polymorphisms (SNPs) (A) are two base-pair variations in the genomes of the human population (B) are genetic markers used to study the genetic basis for disease (C) are large nucleotide differences among individuals located in coding and non-coding sequences in the genome (D) can not be the molecular basis for different alleles (E) All of the listed responses are correct.

29. The most effective molecule for nitrogenous waste disposal in desert animals would be __________. (A) uric acid because it requires very little water for excretion (B) ammonia because it is the most soluble of all the nitrogen-containing metabolic waste products (C) ammonia because it uses less energy to make than uric acid or urea (D) urea because it is less toxic than uric acid (E) uric acid because it takes less energy to make than urea

30. Prions are __________ that are thought to cause disease by __________. (A) DNA molecules ... jumping around the genome and mutating genes (B) mutant DNA molecules ... encoding toxic proteins (C) abnormally shaped proteins ... inducing similar but normally shaped proteins in the brain to adopt the abnormal form (D) RNA molecules ... encoding toxic proteins (E) an abnormal type of capsid ... dramatically enhancing the rate of viral infection

31. In fungi, the function of the mycelium is (A) defense (B) dispersal to distant habitats (C) surviving a period of food shortage (D) obtaining food (E) movement

32. Ancient cyanobacteria were very important in the history of life because they (A) were the first truly multicellular organisms (B) were probably the first organisms to live on Earth (C) are the oldest known prokaryotes (D) extracted heat from the atmosphere, cooling Earth (E) produced atmospheric oxygen

33. The avian flu virus H5N1 is considered a greater long-term threat than the swine flu virus H1N1 because __________. (A) it has a significantly higher mortality rate (B) H5N1 has a greater tendency to become a prophage (C) more people eat chicken meat than pork (D) it is more readily transmitted between human hosts (E) H5N1 is a DNA virus and the similarity of genome chemistry can promote greater virulence and therefore the likelihood of death in humans.

34. To repair a thymine dimer by nucleotide excision repair, in which order do the necessary enzymes act? (A) endonuclease, DNA polymerase I, DNA ligase (B) exonuclease, DNA polymerase III, RNA primase (C) DNA ligase, nuclease, helicase (D) helicase, DNA polymerase I, DNA ligase (E) DNA polymerase I, DNA polymerase III, DNA ligase

35. Which of the following best illustrates homeostasis? (A) All organs are composed of the same four kinds of tissue. (B) Elevated blood CO2 stimulates faster breathing, which rids the body of excess CO2. (C) All the cells in the body have much the same chemical composition. (D) The lung has a large surface for exchange of gases. (E) DNA stores the genetic information in a cell.

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