試題詳解

28. PNGase F is the most effective enzyme for removing N-linked oligosaccharides from glycoproteins/glycopeptides. The glycan removal is accompanied by deamidation of the asparagine side-chain to which the N-linked oligosaccharides are attached. Which of the following statements is false about the target protein after PNGase F treatment?
(A) The molecular weight of the target protein will be reduced.
(B) The extinction coefficient at 280 nm of the target protein will be unchanged.
(C) The primary structure of the target protein will be the same.
(D) The target protein will gain additional aspartic acid residues.
(E) The isoelectric point of the target protein will be reduced.