29. Which of the following restriction enzymes when used to completely digest an E. coli genomic DNA sample would generate DNA fragments in average size of 256 bp.
(A) Not I: GC/GGCCGC
(B) Hind III: A/AGCTT
(C) Hpa II: C/CGG
(D) Hpa I: GTT/AAC
(E) Alul AG/CT

複選題25. Which of the following amino acids is basic in the physiological condition? (A) Aspartic acid (B) Asparagine (C) Tryptophan (D) Lysine (E) Arginine

複選題26.Choose the CORRECT statement describing peptide bond in a polypeptide (A) It has the resonance of π electrons in double bond. (B) It is in planar structure (C) It is freely rotate to give flexibility in stretching. (D) Two peptide bonds have free rotation next to each other. (E) A peptide bond formation involves the conjugation between –COOH and –NH2

複選題27. Which of the following gene transcripts of RNA polymerase II in eukaryotic cells? (A) 45S ribosomal RNA (B) Glyceraldehyde dehydrogenase mRNA (C) (D) U6 snRNA (E) β-actin mRNA

28. E. coli does not express β-galactosidase when its growth medium contains glucose, because (A) CAP is not activated for DNA binding to promote transcription initiation of β-Galactosidase gene. (B) Glucose stimulates the expression of lac repressor. (C) Lac repressor binds to the lac operator sequence. (D) Glucose interferes with lactose binding with the lac repressor. (E) Glucose binds to Lac operator

複選題30. Which of the following amino acids are in the zinc finger motif? (A) Tryptophan (B) Histidine (C) Cysteine (D) Tyrosine (E) Leucine

複選題31. Restrition enzymes cut DNA double helix to generate: (A) 5'-protruding ends (B) 3'-protruding ends (C) blunt ends (D) one 5'-protruding end and one blunt end (E) one 3'-protruding end and one blunt end

32. During mitosis, the nuclear membrane of a cell: (A) disappears temporarily (B) does not disappear (C) disappear permanently (D) disappears partially (E) disappears randomly

複選題33. Mutations in a gene could generate (A) a polypeptide with amino acid changes (B) a polypeptide with no amino acid changes (C) a shorter peptide (D) a longer peptide (E) two shorter peptides

34. Eukaryotic translation of mRNAs is processed in (A) nucleus (B) mitochondria (C) cytosol (D) ER (E) Golgi

35. Which one of the following amino acids can form covalent bonds with other amino acids? (A) lysine (B) valine (C) cystein (D) alanine (E) proline