36. It is crucial that regulation occurs at the pyruvate dehydrogenase because: (A) Pyruvate kinase is reversible. (B) Lactate dehydrogenase is the only other enzyme to use pyruvate. (C) The product acetyl-CoA is committed to oxidation in the citric acid cycle or fatty acid biosynthesis. (D) Alanine aminotransferase would use the pyruvate. (E) All of the above.

1. Compare the two reaction coordinate diagrams below and select the answer that correctly describes their relationship. In eachcase, the single intermediate is the ES complex.Free energy, (A) Diagram (a) describes a strict "lock and key" model, whereas diagram (b) describes a transition-state complementarity model. (B) The activation energy for the catalyzed reaction is #5 in diagram (a) and is #7 in diagram (b). (C) The activation energy for the uncatalyzed reaction is given by #5 + #6 in diagram (a) and by #7 + #4 in diagram (b). (D) The contribution of binding energy is given by #5 in diagram (a) and by #7 in diagram (b). (E) The ES complex is given by #2 in diagram (a) and #3 in diagram (b).

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