試題詳解

40. Which of the following statements correctly describes the process of DNA sequencing by chain termination?
(A) The enzyme DNA polymerase is used to form a primer that binds to the template DNA.
(B) In order to effectively sequence the DNA, the dideoxynucleotides must be present at a higher concentration than the deoxynucleotides.
(C) The use of 4 unique fluorescent labels attached to the dideoxynucleotides allows for a single reaction to be run.
(D) The dideoxynucleotides lack a 5'-OH and therefore can not serve as acceptors in nucleotide addition
(E) Both B and C are correct