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46-49. Edward was performing the cDNA cloning and cDNA inserts with Ndel and BamH1 cloning sites. Several PCR screening the colonies and the plasmid with PCR positive colony was purified and subjected into restriction enzymes, Ndel and Bam H1.digestion assay. The DNA gel electrophoresis was performed to analyze the restriction enzyme digestion result as below.

Lan1, molecular weight standard. Lane 2, no restriction enzymes digestion as control, Lane 3. single enayme Nde 1 digestion., lane 2. Ndel and Bam H1 digestion.

According to the DNA gel data as blow, please answer the questions:


【題組】

49. How many repeated inserts in this construct:
(A) 0
(B) 1
(C) 2
(D) 3
(E) 4



46-49. Edward was performing the cDNA cl..-阿摩線上測驗