5. The benefit of measuring the initial rate of a reaction V0 is that at the beginning of a reaction:
(A) [ES] can be measured accurately.
(B) varying [S] has no effect on V0.
(C) changes in Km are negligible, so Km can be treated as a constant.
(D) V0 = Vmax.
(E) changes in [S] are negligible, so [S] can be treated as a constant.

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