6. Which one of the following amino acids would be considered most polar?
(A) Methionine
(B) Threonine
(C) Leucine
(D) Tryptophan
(E) Proline

7. The major conformation of myoglobin belongs to (A) a-helix (B) ẞ-sheet (C) random coils (D) ẞ-turn (E) none of the above

複選題8. The molecule 2,3-bisphosphoglycerate (BPG) is present in red blood cells in which it binds noncovalently to hemoglobin. Which functional groups in hemoglobin can make the strongest noncovalent interactions with BPG at a pH value of 7.0? (A) sulfhydryl groups (B) Alcoholic hydroxyl groups (C) Hydrocarbon groups (D) Amino groups (E) Carboxy groups

9. A peptide has the sequence Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly, This oligopeptide has a pI close to (A) 4.3 (B) 5.1 (C) 6.0 (D) 7.8

10. Heparin can bind plasma protein antithrombin III. What amino acid residues of an antithrombin III are likely to interact with heparin ? (A) Methionine (B) Serine (C) Lysine (D) Aspartate (E) Tyrosine

11. For an enzyme-catalyzed reaction to reach 90% saturation , the substrate concentration [S] should be (A)(B)(C)(D)(E)

12. For the kinetic scheme shown below, Km can be used to approximate the dissociation constantof ES complex only when  (A) k1 ＜＜ k2 (B)(C)(D)(E) k2 ＜＜ k1

13. Which of the following statements regarding the following reaction scheme is NOT correct? (A) The NH2 group of Ala is transferred directly to KG to form Glu. (B) The binding of KG to active site happens after Pyr release. (C) This is an example of transamination reaction. (D) This scheme represents a ping-pong mechanism. (E) E-CH2NH2 represents a covalently modified enzyme.

14. For a plasmid to be successfully replicated in both E. coli and yeast, this plamid must contain (A) SD (Shine-Dalgarno) and ori sequences (B) CEN and ori sequences (C) T7 promoter and T7 terminator (D) T7 promoter and ori sequence (E) SD (Shine-Dalgarno) and CEN sequences

15. The production of recombinant DNA (DNA cloning) involves the following 4 steps. Please put them into correct experimental order.  I. Insertion of foreign DNA fragment into a cloning vector. II. Preparation of fragments of vector and foreign DNA with compatible ends. III. Introduction of recombinant DNA into a recipient cell for replication. IV. Selection of cells containing the correct recombinant DNA. (A) I→II→III→IV (B) II→I→III→IV (C) II→III→I→IV (D) II→III→I→IV (E) II→I→III→IV

16. Which of the following compounds can mediate the transfer of one-carbon units? (A) thiamin (B) riboflavin (C) nicotinic acid (D) pyridoxal (E) folic acid

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