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108年 - 112 國立清華大學碩士班考試入學試題_生命科學院甲組：生物化學#118786
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2. If an enzyme-catalyzed reaction with a K
M
of 3.5 mM has a velocity of 5 mM/min at a substrate concentration of 0.5 mM, what is the V
max
? (6 %)
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6. Let C be a curve which is the intersection of two surfaces x2+y2=8 and x +z = 3. Find the tangent line to the curve C at the point P(2,2,1) . (10 points)
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7. Explain the fundamental theorem of calculus (two parts in this theorem), no proof is needed. (10 points)
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(A) What is the pI for the following peptide: Ala-Glu-Val-Asp-Lys-Leu? (6%)
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(B) What is the C-terminal amino acid in this peptide? (3%)
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3. This is a cycle of the B-oxidation of saturation fatty acid. Please calculate how many ATP can be generated in a complete oxidation of Myristic-CoA (C14:0-CoA) (if you consider one NADH produces 2,5 ATP, one FADH2 produces 1.5 ATP and one Acetyl-CoA produces 10 ATP)? (10%)
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4. Please explain the mechanism of how F1F0-ATPase produces ATP in mitochondria. You need include the teams of proton gradient, c subunit of F0, and a and B subunits ofF1.(5%)
#506426
5. Please draw a potential energy diagram for the exothermic reaction A→B and explain why the enzyme-catalyzed reaction is faster than the uncatalyzed reaction. (10%)
#506427
6. Please explain why the transmembrane domains of integral membrane proteins mostly have a high secondary structure content. (5%)
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7.what is the key enzyme for the biosynthesis of deoxyribonucleotides? catalytic activity of the enzyme is turned on and off in response to need for dNTPs? general, this reaction involved oxidation-reduction cycle through two re gulatory enzymes which deliver reducing equivalents. Please write names of these two enzymes? (10 %)
#506429
8.How does the completion of human genome project contribute to curr proteomic studies? (5 %)
#506430
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