2. The following data were obtained in a study of an enzyme known tofollow Michaelis- Menten kinetics: (3%)V0 Substrate added(mol/min) (mmol/L)—————————————217 0.8325 2433 4488 6647 1,000—————————————The Km for this enzyme is approximately:

4. Yeast can metabolize D-mannose to ethanol and CO2. In addition tothe glycolytic enzymes, the only other enzyme needed isphosphomannose isomerase, which converts mannose 6-phosphateto fructose 6-phosphate. If mannose is converted to ethanol and CO2by the most direct pathway, which of the compounds and cofactorsin this list are involved? (8%)(A) Lactate (B) Acetaldehyde (C) Acetyl-CoA (D) FAD(E) Glucose 6-phosphate (F) Fructose 1-phosphate (G) Pyruvate(H) Lipoic acid ( I) Thiamine pyrophosphate (J) Dihydroxyacetonephosphate

2. The following data were obtained in a study of an enzyme known tofollow Michaelis- Menten kinetics: (3%)V0 Substrate added(mol/min) (mmol/L)—————————————217 0.8325 2433 4488 6647 1,000—————————————The Km for this enzyme is approximately:

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