申論題

3. You are given 500 ml of a solution at pH 7.5 containing an unknown concentration of nicotinamide adenine dinucleotide (NADH, the molar extinction coefficient of NADH is 6600 M^-¹cm^-1 at 340 nm and pH 7.5). You place 0.5 ml of this solution in a 0.5 cm wide, 0.5 cm long, 2 cm high quartz cuvette and determine the absorbance at 340 nm (A340nın) of this solution to be 0.33. What is the molarity of the NADH solution? How many moles of NADH are in the 500 ml of solution? (6 points)