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研究所、轉學考(插大)◆分子生物學
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101年 - 101 國立清華大學_碩士班入學考試試題_生命科學院甲組、醫學生物科技學程：分子生物學#127971
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4. Describe the basic principle of 2-dimensional gel electrophoresis.
其他申論題
50. 該國的傳統服飾，與當地屬於何種氣候類型有關？
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1. Compare and contrast the mechanisms that prokaryotes and eukaryotes use to find the translation initiation AUG codon.
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2. How does a tRNA serve as an adaptor between the 3-bp codons in mRNA and the amino acids in protein?
#544660
3. Describe the essential enzymatic activities of proteins or RNA in translation elongation and transpeptidation.
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5. Explain the principle of site-direct mutagenesis, and describe a method to carry out this process.
#544663
6. The immediate early/delayed early/late transcriptional switching in the lytic cycle of phage λ is controlled by antiterminators. From all aspects you have learned, what are the differences between N-directed antitermination and Q-directed antitermination during lytic infection by phage λ.
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7. What are the two important elements of a rho-independent (intrinsic) transcription terminator? What is the key element of a rho-dependent terminator?
#544665
(1) Run-off transcription is commonly used to check the efficiency and accuracy of in vitro transcription. Please describe how a run-off assay works.
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(2) The G-less cassette transcription assay is a variation of the run-off assay. Please explain what a G-less cassette transcription assay is.
#544667
(3) Nuclear run-on transcription can be used to measure transcription rates in vivo. Please describe how a nuclear run-on assay works and explain how it differs from a run-off assay.
#544668
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