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23) In relation to protein translation, explain the main reaction for each A site, P site and E site of the ribosome. (5%)
22) How can DNA and RNA be degraded? (DNA = 5%； RNA = 5%)
21) For restriction enzyme digestion, 'sticky end' and 'blunt end' enzymes are used. What is 'sticky end' and 'blunt end' digestion? (5%)
15) For real time qPCR, which of the below Ct values (Cts) indicate the greatest abundance of the target nucleic acid? (A) Cts ＜ 20 (B) Cts of 25-30 (C) Cts of 30-35 (D) Cts of 35-38 (E) Cts of 38-40
14) The ratio of absorbance at 260 nm and 280 nm is used to assess the purity of DNA and RNA. Which of the below values indicates the purest 260/280 ratio of DNA? (A) 1.0 (B) 1.3 (C) 1.8 (D) 2.2 (E) 3.2
13) At which pH is RNA most stable? (A) pH 3-4 (B) pH 4-5 (C) pH 5-6 (D) pH 7-8 (E) All of above

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