3. 如果貴輪預期遇到低氣壓或颱風,身為大副,您會做些什麼樣的應對措施?

2. 請問貴輪若在上題的位置,是否適合指派甲板除鏽油漆工作?理由為何?

三、氣象傳真圖運用:
1. 參考第一頁氣象圖,您擔任某輪大副,貴輪正好位在B點所指示的鋒面上,請問您將遇到什麼樣的天氣?

40. We are studying a transcription factor. What is incorrect? (A) Its mRNA expression is determined by northern blot. (B) Its protein expression is monitored by western blot. (C) Its target gene promoter is cloned from a cDNA library. (D) The promoter is ligated with luciferase gene for reporter assay. (E) The electrophoretic mobility shift assay is performed to display DNA-protein interaction.

39. Induced pluripotent stem (iPS) cells are a type of pluripotent stem cell that are obtained by reprogramming somatic cells through artificial expression of key transcription factors including OCT4, KLF-4, SOX2, and c-MYC. What is incorrect? (A) These transcription factors promote self-renew. (B) They suppress differentiation-related gene expression. (C) They alter DNA sequences to regulate gene expression. (D)DNA methylation inhibitor could facilitate iPS cell formation. (E) Histone deacetylase inhibitor could induce iPS cell formation.

38. Which description is incorrect for chromatin-immunoprecipitation (ChIP) experiment? (A) It is for DNA-protein interaction. (B) Formaldehyde is used to crosslink DNA-protein only. (C) The genomic DNA has to shear into smaller fragments by sonication or nuclease digestion. (D) The specific antibody against the interested protein is to immunoprecipitate cross-linked DNA fragments. (E) The precipitated DNA regions are examined by quantitative PCR.