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4. Draw the chemical structure of the following compounds (2 points for each): (A) urea (B) dATP
(C) aspartate
3. You are given 500 ml of a solution at pH 7.5 containing an unknown concentration of
nicotinamide adenine dinucleotide (NADH, the molar extinction coefficient of NADH is 6600
M¹cm²¹ at 340 nm and pH 7.5). You place 0.5 ml of this solution in a 0.5 cm wide, 0.5 cm long,
2 cm high quartz cuvette and determine the absorbance at 340 nm (A340nın) of this solution to be
0.33. What is the molarity of the NADH solution? How many moles of NADH are in the 500 ml
of solution? (6 points)
2. The replisome is a complex molecular machine that carries out DNA replication. Please write
down three enzymes are included in the replisome. (9 points)
35. Which amino acid is complexed to iron-sulfur clusters to enable them to associate with proteins?
(A) methionine (B) cysteine (C) serine (D) lysine (E) tyrosine.

二、簡答題(共30分)
34. Which of the following reactions is not located in the mitochondria? (A) tricarboxylic acid cycle (B)
cholesterol biosynthesis (C) ketone body synthesis (D) ẞ-oxidation (E) electron transport and
oxidative phosphorylation.
33. Triton X-100 is a nonionic detergen that denatures proteins by disrupting which of the following?
(A) hydrogen bonds (B) disulfide bridges (C) hydrophobic interactions (D) salt bridges (E) covalent
bonds.

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