I.專業英文填空:請參考下列答案庫,限用英文,每題2分、文法錯誤扣1分(30%)。
※注意:請於試卷内之「非選擇題作答區」作答,並應注明作答之題號。
答案庫(含單數名詞、原型動詞、形容詞與副詞):
Biological reactions __(14)__ by enzymes are generally very __(15)__ to certain chemicals
known as the __(16)__ of the enzymes; the chemicals bind to the __(17)__ . site of the enzyme to
become an ES complex with a much lower __(18)__ energy.
The reaction therefore can occur under mild conditions (e.g. temperature and pressure) with sufficient
efficiency.Based on Michaelis-Menten model, the reaction rate is related to the concentration of ES complex
as the following__ (19)__ kinetics.
In the__ (20)__ state, the concentration of ES complex can be solved as follows.
An enzymatic reaction rate therefore increases non-linearly with the substrate concentration and reaches a maximum as the substrate concentration being much higher than __(21)__.
Since the kinetics is not linear, it will be more convenient to analyze the parameters by plotting the
__(22)__values of the reaction rate against those of the substrate concentration.
The __(23)__ of the plot will be proportional to the Michaelis constant.
The following plots show the existence of__ (24)__ . inhibitors which will alter the apparent KM (the
slope of the plot) of the enzyme kinetics without changing the Vmar. The higher the inhibitor concentration, the__(25)__ the apparent KM.
According to Arthenius expression, the rate constant (k2) of an enzyme will be alfected by the temperature (T)
and the activation energy (Ea). 'The reaction for the substrate possesses an Ea _ __(26)__ than other
of the enzyme.chemicals, which is the origin of the substrate __(27)__of the enzyme.
The best way to calculate the activation energy is to multiply the slope of the semi-logarithmic plot of V
against (1/T) with the negative value of __(28)__.