30. What the following statements for enzyme is/are TURE:
(A) Catalytic activity and substrate binding activity can not be separated
(B) Catalytic function of an enzyme mostly relies on the charged group on the amino acid side chains
(C) In Michaelis-Menten curve, same amount of enzyme with defects in substrate binding can never reach to the same Vmax as wild-type
(D) The velocity (V) of catalysis of an enzyme is constant and it is independent of substrate concentration
(E) Dissociation constant (Kd) can be used to represent the binding affinity between enzyme and substrate