104年 - 台灣大學 104 碩士班研究所生物化學C#82427

科目：研究所、轉學考(插大)◆生物化學 | 年份：104年 | 選擇題數：0 | 申論題數：51

試卷資訊

所屬科目：研究所、轉學考(插大)◆生物化學

選擇題 (0)

申論題 (51)

1. The fluorescence of green fluorescent protein (GFP) Is derived from the chemical bond rearrangement and oxidation of the sequence Ser-Tyr-Gly. Please draw the molecular structure of Ser-Tyr-Gly. (3 points)
a) What kind(s) of the residue(s) in this novel peptide can possibly be modified by phosphorylation? (1 point)
b) What kind(s) of the residue(s) in this novel peptide can possibly be modified by ubiquitination? (1 point)
c) What kind(s) of the residue(s) in this novel peptide can possibly be modified by O-linked glycosylation? (1 point)
d) What kind(s) of the residue(s) in this novel peptide can possibly be modified by N-Iinked glycosylation? (1 point)
e) What kiad(s) of the residue(s) in this novel peptide can possibly be modified by disulfide bond? (1 point)
f) When this peptide is fully hydrolyzed by HC1 into free amino acids, which amino acid has the highest absorbance at 280 am? (1 point)
g) When this peptide is fully hydrolyzed by HC1 into free amino acids, which amino acid can obtain bright yellow ninhydrin products? (1 point)
a) One NAD+ can accept ___________electron(s) to become an NADH. Please fill in the number in the sentence above. (1 point)
b) Among (A) (B) (C) (D) (E) indicated on NAD+ which site can accept electron(s) to become NADH? (1 point)
c) Among (A) (B) (C) (D) (E) indicated on NAD+. which site can accept a phosphate group to become NADP+? (1 point)
d) NADH can be distinguished from NAD+ by analyzing them with a spectrophotometer at________nm. (1 point)
a) Which enzyme is in charge of the specific proteolytic cleavage of prothrombin? (1 point)
b) Which enzyme is in charge of the specific proteolytic cleavage of trypsinogen? (1 point)
c) Which enzyme is in charge of the specific proteolytic cleavage of chymotrypsinogen? (1 point)
d) Which enzyme is in charge of the specific proteolytic cleavage of fibrinogen? (1 point)
e) What kind of amino acid is the major active site found in the enzymes mentioned above? (1 point)
a) Amino groups (1 point)
b) One-carbon.groups (1 point)
c) Aldehydes (1 point)
d) C〇2(l point)
6. Please briefly describe the “lock and key” model and the “induced fit” model for enzyme-substrate interactions. (4 points)
a) Please give the definitions of kd. (1 point)
b) Please define the association constant by using ka and  kd. (1 point)
c) Please define the dissociation constant Kd by using Ka. (1 point)

d) Please give the definition of Kd by using θ and [L]. (2 points)

8. What is the substrate which is required for initiation of the reaction indicated below? (1 point)5e27ea989b34f.jpg

9. The restriction enzyme EcoRV can recognize the specific sequence GATATC and provide a blunt end digestion. Among the covalent bonds of (a) (b) (c) (d) (e) (I) (g) (h), which bond(s) can be digested by EcoRV? (2 points)5e27eaae102c4.jpg

10. Please draw the molecular structure of p-D-galactopyranosyl-(l→4)-α-D-glucopyranose. (2 points)

11. The molecular structure of D-glucose in the Fisher projection format is shown as below. Please draw the molecular structures of D-mannose and D-fructose in the Fisher projection format. (2 points)5e27eaf39fe27.jpg

12. 丁he molecular structures of galactose and N-acetylneuraminic acid are shown as below. Please draw the α-2, 3-linked and  α-2, 6-linked conjugations, composed by these two sugars, and point out which one can be recognized by the human influenza virus. (3 points)5e27eb12a4749.jpg
13. Which type of fatty acid contains a double bond (C:C) at the third carbon atom from the distal end of the fatty acid? (1 point)

14. The diagram shows a simple illustration of the molecular structure of phosphatidylcholine. Please answer which molecules should be filled in the positions of C, D and E. (3 points)5e27eb3e8d574.jpg
15. Regarding the signal transduction pathway, how does binding of epinephrine initiate the cAMP production? Discuss briefly in terms of receptor structure and functions. (4 points)
16. What is metabolomics? What are the principal tools for metabolomic analyses? (4 points)
17. Please describe how sucrose is synthesized in plants (5 points) and how it is catabolized in mammals. (5 points)
18. Please compare the “Q cycle” in mitochondria and in chioroplasts (6 points)
19. Please briefly describe the role of vitamin Bn in the metabolism of odd-number fatty acids. (3 points)
20. How is the Calvin cycle regulated? (5 points)
21. How are triacylglycerols mobilized from the adipose tissues? (5 points)
22. How is the urea cycle linked to the citric acid cycle? (5 points)
a) pyruvate (1 point)
b) fumarate (1 point)
c) acetoacetyl CoA (1 point)
a) Nitric oxide (1 point)
b) Pyrimidine ring (2 points)
c) Heme (2 points)
d) Prostaglandins (1 point)
e) Progesterone (1 point)
f) Glycogen (1 point)
g) Starch (1 point)