107年 - 107 長庚大學_研究所碩士班招生考試試題_生物醫學研究所碩士班：生物化學#126157

1. Lipoprotein lipase acts in_____.(A) hydrolysis of triacylglycerols of plasma lipoproteins to supply fatty acids to various tissues.(B) intestinal uptake of dietary fat.(C) intracellular lipid breakdown of lipoproteins.(D) lipoprotein breakdown to supply needed amino acids.

2. Which of the following enzyme in glycolysis requires ATP as a substrate?(A) Hexokinase(B) Glyceraldehyde-3-phosphate dehydrogenase(C) Pyruvate kinase(D) Aldolase

3. In the alcoholic fermentation of glucose by yeast, thiamine pyrophosphate is a coenzyme required by__________.(A) aldolase.(B) hexokinase.(C) lactate dehydrogenase.(D) pyruvate decarboxylase.

4. Common features found in a cloning plasmid used for protein expression include all EXCEPT which of the following? (A) Telomeric ends (B) Origin of replication. (C) Antibiotic resistance marker(s) (D) Ribosome binding site

5. The PCR reaction mixture does NOT include_________.(A) all four deoxynucleoside triphosphates.(B) DNA containing the sequence to be amplified.(C) DNA ligase.(D) heat-stable DNA polymerase

6. A genetic inherited disease is caused by mutation in medium-chain acyl-CoA dehydrogenase, and which of the following pathway is affected by this enzyme? (A) beta-oxidation (B) glycolysis (C) Lipid synthesis (D) Lipid storage

7. Which lipoprotein is generated by liver?(A) IDL(B) VLDL(C) LDL(D) Chylomicron

8. which is regulated by insulin in fatty acid synthesis?(A) Activation of cAMP(B) Activation of pyruvate carboxylase(C) Activation of citrate lyase(D) Activation of propionyl CoA carboxylase

9. Which of the following component involves in detoxification?(A) CO2(B) cytochrome P-450(C) cytochrome C(D) desaturase

10. The degradation of HMG CoA reductase is mediated through?(A) Acetylation(B) Phosphorylation(C) Ubiquitination(D) Prenylation

11. which of the following statement is right?(A) The main source of ATP in red striated muscle is from fatty acid oxidation(B) The main source of ATP in white striated muscle is from fatty acid oxidation(C) There are a lots of mitochondria in white striated muscle(D) The muscle contraction is slower in white than in red striated muscle

12. Histones and DNA have a strong physical interaction because__________.(A) DNA is negative charged and histones are positively charged.(B) Both proteins share common charges.(C) Both DNA and proteins are hydropohobic.(D) Both DNA and proteins are hydrophilic

13. In eukaryotic cells, which one of the following statement about DNA replication is true.(A) There are multiple origins of replication that function consecutively.(B) There are multiple origins of replication that function simultaneously.(C) DNA replication proceeds unidirectionally from many origins(D) The sequences of replication origin is highly conserved from yeast to human.

14. Which DNA component is essential for a chromosome to be maintained in cells from mother cells to daughter cells?(A) centromere(B) telomere(C) origin of replication(D) all of above

15. Which one of method is NOT suitable to analyze DNA-protein interaction?(A) DNase-I footprint assay(B) Gel shift assay(C) Chromatin-immunoprecipitation assay(D) Southern blot analysis

16. DNA inversion in genome is difficult to detect because it________.(A) never visibly changes the chromosome banding pattern(B) changes recombination frequency(C) does not usually cause an abnormal phenotype(D) normally is removed immediately from the nature population

17. The primary function of basal transcription factors is to________.(A) assist in associating RNA polymerase with the promoter element.(B) Increase transcription by binding enhancer sequences(C) activate RNA synthesis at origin of replication(D) regulate activity of RNA polymerase I

18. Post-translational modification regulates protein activity. Which one of the following modification is also observed for genomic DNA? (A) ubiquitination (B) methylation (C) sumorylation (D) glycosylation

19. Which of the following coenzymes is NOT a carrier of electrons in biological redox reactions?(A) Coenzyme A(B) Niacin.(C) Riboflavin.(D) Transition metal ions, such as those of iron or copper.

20. The immediate electron acceptor for the majority of the oxidative reactions of the citric acid cycle is______.(A) ATP.(B) NAD.(C) FAD.(D) coenzyme A.

21. Complex I of the electron transport chain oxidizes________，reduces_________，and______protons in the process.(A) FADH2, coenzyme Q, doesn't pump(B) FADH2, coenzyme Q, pumps(C) NADH, coenzyme Q, doesn't pump(D) NADH, coenzyme Q, pumps

22. Chymotrypsin is a protease, an enzyme catalyzes the hydrolytic cleavage of______bonds.(A) hydrogen(B) polypeptide(C) disulfide(D) carbonyl

23. Chymotrypsin is specific for bonds adjacent to aromatic amino acid residues. Which is NOT the substrate of chymotrypsin? (A) Phenylalanine (B) Tyrosine (C) Tryptophan (D) Histidine

24. What material plays a role of radical initiator in formation of sodium dodecyl sulfate (SDS) polyacrylamide gel? (A) Ammonium persulfate (B) Tetramethylethylenediamine (C) SDS (D) Acrylamide

25. Which of the following method can NOT be used to study the sequence of monosaccharides, position and configuration of glycosidic bonds? (A) Hydrolysis with strong acid (B) Enzymatic hydrolysis with specific glycosidases (C) NMR (D) mass spectrometry

26. Which method is NOT the common way to denature a protein?(A) by miscible organic solvents(B) by extremes of pH environments(C) by urea(D) by filtration

27. The information needed for the structure of a protein is determined by__________.(A) Amino acid composition(B) Primary structure(C) Secondary structure(D) Tertiary structure

28. The molecular weight of protein or peptide can be determined by__________.(A) Isoelectric focusing electrophoresis (IEF)(B) Ion-exchange liquid chromatography(C) Mass spectrometry(D) Circular dichroism (CD)

29. Which one described below is NOT true for protein structure?(A) Hydrogen bonds are most important in secondary structure of protein.(B) Reverse turn is often found connecting the strands of an antiparallel ẞ-sheet.(C) The overall folding of a single protein subunit is called quaternary structure.(D) A domain is a folded region of protein, often shared by proteins and has specific biological function.

30. Which one described below is true for post-translational modification (PTM) of proteins?(A) Phosphorylation but not dephosphorylation plays an important role in signal transduction.(B) The activity of histone can be regulated by methylation but not acetylation at its lysine residue.(C) The conformation and also biological activity of protein can be regulated by PTM.(D) Ubiquitination would decrease the molecular weight (size) of protein.

31. Which one of the following statements is true for protein misfolding?(A) May increase the protein stability(B) May reduce the water solubility of protein.(C) Would easily aggregate in the cytoplasm.(D) All of the above.

32. Which of the following is true of the lagging strand during DNA replication?(A) It is made in the same direction as the replication fork in opening.(B) It contains Okazaki fragments.(C) It is made much more slowly than the leading strand.(D) It is made in the 3' to 5' direction.

33. Which of the following statements about epigenetic modifications are correct?
a. Epigenetic modifications are not influenced by underlying DNA sequence
b. Epigenetic modifications are stable over time
c. X-chromosome inactivation occurs in female mammal only
d. DNA methylation always negatively correlated with gene expression on the promoter regions of a gene.
e. Epigenetic modifications can be influenced by the environmental factors, such as nutrition.(A) a, b, d, e(B) e only(C) a, b, c, d, e(D) a, d, e

34. HOW MANY of the following techniques can be used to detect mRNAs?
a. Northern blotting b. In situ hybridization c. Real-time PCR d. Sanger sequencing(A) 1(B) 2(C) 3(D) 4

35. Which of the following statement is wrong about alternative splicing? (A) Alternative splicing is a process that exons of a gene are differentially joined or skipped (B) Multiple protein isoforms can be encoded by a single gene during the process (C) The process can produce two or more proteins that differ in their function (D) The alternatively splicing events are often differentially regulated among individuals and populations, but not across tissues

36. Which buffer molecule does NOT carry a positive charge?(A) Tris(B) phosphate(C) imidazole(D) none of the above

37. Which of the following enzymes is NOT responsible for assimilation of ammonia?(A) glutamine amidotransferase(B) glutamate dehydrogenase(C) glutamate synthase(D) carbamoyl phosphate synthetase

38. Which symptom is a consequence of vitamin B12 deficiency?(A) anemia(B) homocysteinemia(C) neurological disorder(D) all of the above

39. Which enzyme is defective in patients with maple syrup urine disease?(A) branched-chain a-amino acid aminotransferase(B) branched-chain a-amino acid dehydrogenase(C) branched-chain a-keto acid dehydrogenase(D) branched-chain a-keto acid decarboxylase

40. 2',3'-dideoxycytidine (ddC), can serve as a drug for HIV infections because of the absence of a_____group which is required for______.(A) 2'-hydroxyl; transcription(B) 3'-hydroxyl; replicative chain elongation(C) 5'-hydroxyl; translation(D) 2'-hydroxyl; replicative chain elongation