所屬科目：研究所、轉學考(插大)◆生物化學
1. There are a total of ______ “sense” codons in the genome. (A) 4 (B) 16 (C) 61 (D) 64 (E) 72.
2. B form DNA has a pitch (height per turn) of (A) 0.10 (B) 0.15 (C) 0.34 (D) 0.54 (E) 3.4 nm.
3. Which of the following descriptions about Hb (hemoglobin) is incorrect ? (A) One Hb can bind one O₂ molecules (B) HbA has an α₂β₂ structure (C) HbF has an α₂γ₂ structure (D) HbA has a higher affinity for BPG than does HbF (E) CO₂ reduces the binding affinity of Hb for O₂.
4. Which of the following descriptions about the Michaelis-Menten rate equation is incorrect ? (A) KM measures the substrate concentration at which the reaction rate is Vmax/2 (B) kcat is the turnover number that measures the rate of the catalytic process (C) The ratio kcat/KM is a convenient measure of enzyme efficiency (D) kcat = Vmax/[Enz] (E) A competitive inhibitor decreases the apparent KM.
5. To visualize protein bands on SDS-PAGE, ______ is commonly used to stain proteins after gel electrophoresis. (A) Coomassie Brilliant Blue (B) EtBr (C) NADH (D) CNBr (E) BUdR.
6. A DNA segment of 100 base pairs in the A form. What is its approximate molecular weight?_______kD. (A) 33 (B) 65 (C) 330 (D) 650 (E) 3,300
7. Absorbance at ______ nm is frequently used to determine the concentration of RNA. (A) 200 (B) 260 (C) 280 (D) 400 (E) 540.
8. Which of the following compounds has the highest molecular weight? (A) adenine (B) adenosine (C) adenylate (D) uracil (E) uridine.
9. Which of the following descriptions regarding disaccharide is incorrect? (A) Sucrose is α-D-glucopyranosyl (1→4) β-D-fructofuranoside (B) Sucrose is a nonreducing sugar (C) Maltose has an α(1→4) linkage (D) Cellobiose is β-D-glucopyranosyl (1→4) β-D-glucopyranose (E) Lactose is a reducing sugar.
10. Which of the following restriction endonucleases is an isoschizomer of BglII (AGATCT)? (A) EcoRI (GAATTC) (B) SpeI (ACTAGT) (C) BamHI (GGATCC) (D) SalI (GTCGAC) (E) EagI (CGGCCG).
11. Which scientist made a significant contribution to the understanding of collagen structure? (A) K. B. Mullis (B) J. D. Watson and H. C. Crick (C) A. D. Hershey and M. Chase (D) S. B. Prusiner (E) G. N. Ramachandran.
12. Tween 20 is a nonionic surfactant that denatures proteins by disrupting ______? (A) hydrogen bonds (B) disulfide bridges (C) hydrophobic interactions (D) salt bridges (E) covalent bonds.
13. How many stereoisomers for a pentose? (A) 2 (B) 4 (C) 6 (D) 8 (E) 16.
14. The chemical bond between ribose and adenine in AMP is a(n) ______ bond. (A) ether (B) ester (C) amide (D) aldehyde (E) glycosidic.
15. Which of the following lipids is not a major component of cellular membranes? (A) glycerophospholipids (B) sphingolipids (C) triacylglycerol (D) glycosphingolipids (E) glycoglycerolipid.
16. TψC loops are found in (A) mRNA (B) rRNA (C) tRNA (D) hnRNA (E) snRNA.
17. A tRNA molecule, on average, contains ______ nucleotides. (A) 30 (B) 50 (C) 67 (D) 76 (E) 100.
18. Which scientist made a significant contribution to the understanding of prions? (A) K. B. Mullis (B) J. D. Watson and H. C. Crick (C) A. D. Hershey and M. Chase (D) S. B. Prusiner (E) F. Sanger.
19. Which of the following amino acids contains only one codon? (A) Ala (B) Lys (C) Tyr (D) Met (E) Arg.
20. The Western blotting method is commonly used for detection of (A) proteins (B) DNA (C) RNA (D) lipids (E) carbohydrates.
21. The Northern blotting method is commonly used for detection of (A) proteins (B) DNA (C) RNA (D) lipids (E) carbohydrates.
22. Which of the following tautomeric forms is the form of fructose in sucrose? (A) α-pyranose (B) β-pyranose (C) α-furanose (D) β-furanose (E) all of the above.
23. The ribosome-binding sequence is ______ in E. coli? (A) AATT (B) AAGG (C) ATGC (D) TTCC (E) TTGG.
24. Azide is poisonous, because it tightly binds ______ of the respiratory chain. (A) CoQ (B) cyt a (C) cyt b (D) cyt c (E) cyt a₃
25. Which pair of enzymes listed below generates NADPH? (A) glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (B) malic enzyme and glucose-6-phosphate dehydrogenase (C) citrate lyase and malic enzyme (D) 6-phosphogluconate dehydrogenase and fructose-bisphosphatase-1 (E) fructose-bisphosphatase-1 and hexose kinase.
26. Which pair of enzymes listed below is unique to the glyoxylate cycle? (A) pyruvate dehydrogenase and fructose-bisphosphatase-1 (B) pyruvate carboxylase and 3-phosphoglycerate kinase (C) PEPCK and pyruvate carboxylase (D) citrate lyase and malate synthase (E) pyruvate carboxylase and phosphofructokinase-1.
27. The major monosaccharides in coke are (A) glucose (B) fructose (C) galactose (D) glucose and fructose (E) galactose and fructose.
28. Which of the following reactions is not located in mitochondria? (A) tricarboxylic acid cycle (B) electron transport (C) glycolysis (D) $\beta$-oxidation (E) oxidative phosphorylation.
29. In a Lineweaver-Burk double reciprocal plot, the intercept of the y-axis equals ______. (A) $K_{\mathrm{M}}$ (B) - $1 / K_{\mathrm{M}}$ (C) $V_{\max}$ (D) $1 / V_{\max}$ (E) $k_{\mathrm{cat}} / K_{\mathrm{M}}$.
30. Phosphofructokinase-1 (A) is activated by ADP and citrate (B) is inhibited by ATP and citrate (C) is activated by citrate and fructose-2,6-bisphosphate (D) is activated by ATP and fructose-2,6-bisphosphate (E) is inhibited by ATP and fructose-2,6-bisphosphate
31. All $\alpha$-amino acids except ______ contain a chiral $\alpha$-carbon. (A) Gly (B) Ala (C) Ile (D) His (E) Met.
32. What is the size of the human genome? (A) $3 \times 10^{7}$ (B) $3 \times 10^{8}$ (C) $3 \times 10^{9}$ (D) $3 \times 10^{10}$ (E) $3 \times 10^{11}$ bp.
33. How many moles of NADH molecules are produced in the TCA cycle per molecule of acetyl-CoA oxidized? (A) 5 (B) 4 (C) 3 (D) 2 (E) 1.
34. Waxes are formed by esterification of ______ and ______? (A) fatty acids and glycerols (B) fatty acids and glucose (C) fatty acids and alcohols (D) fatty acids and glycerol-3-phosphate (E) fatty acids and sphingosine.
35. Which carbons are released first as $\mathrm{CO}_{2}$ in the metabolism of glucose to $\mathrm{CO}_{2}$? (A) carbons 1 and 2 (B) carbons 3 and 4 (C) carbons 5 and 6 (D) carbons 1 and 6 (E) none of the above.
36. Which of the following scientists demonstrated that DNA, rather than proteins, is the genetic material? (A) F. Sanger (B) J. D. Watson and H. C. Crick (C) A. D. Hershey and M. Chase (D) S. Altman and T. Cech (E) S. B. Prusiner.
37. All of the following are paired with their preferred substrate except: (A) brain: fatty acids (B) heart: fatty acids (C) anaerobic skeletal muscle: glucose (D) red blood cell: glucose (E) adipose tissue: fatty acids.
38. ______ is considered an adapter molecule that links an amino acid to its codon(s). (A) mRNA (B) rRNA (C) tRNA (D) ssRNA (E) miRNA.
39. ______ within the ribosome is responsible for polypeptide synthesis. (A) aminoacyl-tRNA synthetase (B) met-aminopeptidase (C) degradase (D) ubiquitin (E) peptidyl transferase.
40. Which tautomeric form of ribose predominates in solution? (A) $\alpha$-pyranose (B) $\beta$-pyranose (C) $\alpha$-furanose (D) $\beta$-furanose (E) all of the above.
阿摩線上測驗
登入
阿摩線上測驗
登入