108年 - 108 亞洲大學_學士後招生考試_獸醫學系：生物化學#101303

32. Which description is NOT correct? (A) The complete oxidation of palmitate (COOH) yields 106 molecules of ATP. (B) Animals can convert fatty acids into glucose directly. (C) Fatty acids are synthesized in the cytoplasm. (D) Intermediates in fatty acid synthesis are covalently linked to the sulfhydryl groups of an acyl carrier protein (ACP).

33. Which of the following receptor plays a key role in controlling cholesterol metabolism? (A) VLDL receptor (B) IDL receptor (C) LDL receptor (D) HDL recepto

34. Which of the following hormone is NOT derived from cholesterol? (A) Insulin (B) Progesterone (C) Androgens (D) Estrogens

35. Which description of cytochrome P450 is correct? (A) Steroids are hydroxylated by cytochrome P450 monooxygenases that use NADPH and O2. (B) The cytochrome P450 system, which in mammals is located primarily in the smooth endoplasmic reticulum of the liver and small intestine. (C) The cytochrome P450 system metabolize ethanol. (D) All of the above.

36. Which of the following compound is NOT involved in urea cycle? (A) Glutamate (B) Ornithine (C) Fumarate (D) Aspartate

37. Which of the following coenzyme is required for aminotransferase? (A) Folic acid (B) Biotin (C) Flavin adenine dinucleotide (D) Pyridoxal phosphate

38. Which of the following coenzyme is required for DNA synthesis? (A) Folic acid (B) Biotin (C) Flavin adenine dinucleotide (D) Pyridoxal phosphate

39. Which description of ubiquitin is NOT correct? (A) Ubiquitin is highly conserved in eukaryotes and prokaryotes. (B) Ubiquitin attached to the ε-amino groups of several lysine residues on a protein destined to be degraded. (C) The energy for the isopeptide bonds of ubiquitination comes from ATP hydrolysis. (D) Three enzymes (E1, E2 and E3) participate in the attachment of ubiquitin to a protein.

40. Which description of CRISPR/Cas9 is correct? (A) CRISPR/Cas9 is a novel protein metabolic pathway. (B) CRISPR/Cas9 is a novel coenzyme. (C) CRISPR/Cas9 is a novel protein purification technology. (D) CRISPR/Cas9 is a novel technology that can be used to edit genes within organisms.

41. Mitochondria are the primary sources of energy for cells, organs, and the living animal. Which enzyme is NOT contained in this organelle? (A) Succinate dehydrogenase (B) Fumarase (C) Cytochrome oxidase (D) Rubisco

42. Which of the following ion plays a key role in extracting electrons from H2O to form O2 in photosystem II of photosynthesis? (A) Calcium (B) Natrium (C) Manganese (D) Iron

複選題
43. Metabolism produces both inorganic and organic acids. Which metabolism produce(s) are nonvolatile and excreting via the kidney? (A) Lactic acid (B) Acetoacetic acid (C) β-hydroxybutyrate (D) All of the above

44. Which of the following aqueous solutions has the lowest pH? The solutions have H+ concentration respectively of (A) mol/L (B) mol/L (C) mol/L (D) mol/L

45. Acetic acid (CH₃COOH) has a pKa of 4.76. What is the molar ratio of potassium acetate (CH₃COOK) to acetic acid at pH 4.76? (A) 1:1 (B) 2:1 (C) 10:1 (D) 1:10

46. Which amino acids are relatively nonpolar (hydrophobic)? (A) Phe, Tyr, Trp (B) Ser, Asn, Gln (C) Lys, Arg (D) Asp, Glu

47. Which amino acids have positive charge at pH 7.0? (A) Phe, Tyr, Trp (B) Ser, Asn, Gln (C) Lys, Arg (D) Asp, Glu

48. N-linked glycosylation is important process for both the structure and function of some eukaryotic proteins. Which amino acid plays a key role in this process? (N-glycans are attached to the nitrogen atom of this amino acid) (A) Pro (B) Trp (C) Lys (D) Asn

49. Which amino acid has a significant effect on the tertiary structure of the protein? (A) Pro (B) Trp (C) Lys (D) Asn

50. The indole group absorbs UV light at 280 nm. It is useful for spectrophotometric measurement of protein concentration. Which amino acid has an indole ring? (A) Pro (B) Trp (C) Lys (D) Asn

51. Which amino acids carry a negative charge at pH 7.0? (A) Arg, Lys (B) Phe, Try, Trp (C) Glu, Asp (D) None of the above

52. Which of the following pairs bonds can rotate within a peptide backbone and the torsion angle of the latter assigned as phi angle? (A) N-Cα and Cα-CO (B) Cα-CO and N-Cα (C) CO-N and N-Cα (D) N-Cα and CO-N

53. Which one is NOT a component of inner mitochondrial membrane? (A) Cytochrome c (B) NADH dehydrogenase (C) Phosphatidylcholine (D) Deoxyribonucleic acid

54. During the separation of protein molecules, which method relates to the isoelectric point (pI) of the protein? (A) Immunoprecipitation (B) SDS-PAGE (C) Ion exchange (D) Gel filtration

55. During the separation of protein molecules, which method is related to protein denaturation? (A) Immunoprecipitation (B) SDS-PAGE (C) Ion exchange (D) Gel filtration

56. Which nucleosides below contain both pyrimidines? (A) A, T (B) C, T, U (C) C, G (D) A, G, U

57. What force drives the formation of protein tertiary structure? (A) Van der Waals force (B) Electrostatic interaction (C) H-bonds (D) All of the above

58. When a polymer of 36-residue forms an α-helix, how many repeats would you expect it to be? (A) 7 (B) 8 (C) 9 (D) 10

59. What type of inhibitor X is this Lineweaver–Burk plot shown?
(A) Competitive inhibition (B) Uncompetitive inhibition (C) Noncompetitive inhibition (D) Irreversible inhibitors

60. What type of inhibitor Y is this Lineweaver–Burk plot shown?
(A) Competitive inhibition (B) Uncompetitive inhibition (C) Noncompetitive inhibition (D) Irreversible inhibitors