110年 - 110 國立中央大學_碩士班招生考試_生命科學系/生物醫學組(一般、在職生):生物化學#105354

1.How many different codons are normally used to decode the amino acid "tryptophan" in the genome of E. coli? (A) 0 (B) 1 (C) 2 (D) 4 (E) 6.

2.A DNA (B form) has a pitch (height per turn) of (A) 0.15 (B) 0.54 (C) 0.34 (D) 3.4 (E) 10 nm.

3.Which of the following descriptions about Hb (hemoglobin) is incorrect? (A) One Hb can bind 1 O₂ molecules (B) HbA has an a₂β₂struc cture (C) HbF has an a₂γ₂ structure (D) HbF has a lower affinity for BPG than does HbA (E) CO₂decreases the binding affinity of Hb for O₂.

4.Which of the following descriptions about the Michaelis-Menten rate equation is incorrect? (A) K_M measures the substrate concentration at which the reaction rate is (B) is the turnover er number that measures the rate of the catalytic process (C) The ratio is a convenient measure of enzyme efficiency (D) K_M is the Michaelis constant (E) A competitive inhibitor increases the apparent K_M.

5.Which of the following compounds is comn monly used to stain proteins after SDS-PAGE? (A) EtBr (B) Coomassie brilliant blue (C) Cyber Green (D) CNBr (E) BUdR.

6.A single-stranded DNA segment of 1,000 bases in the A form. What is its approximate molecular weight? (A)33 (B) 65 (C) 330 (D) 650 (E) 3,300 kD.

7.Absorbance at ______nm is frequently used to determine the concentration of proteins. (A) 200 (B) 260 (C) 280 (D) 400 (E) 540.

8.Which of the following compounds has the highest molecular weight? (A) adenine (B) adenosine(C) adenylate (D) uracil (E) uridine.

9.Which of the following descriptions regarding disaccharide is incorrect? (A) Sucrose is a-D-glucopyranosyl (1→2) β-D-fructofuranoside (B) Sucrose is a non-reducing sugar (C) Maltose has an a(1→4) linkage (D) Cellobiose is β-D-glucopyranosyl (1→4) β-D-glucopyranose (E) Lactose is a non-reducing sugar.

10.Which of the following restriction endonucleases is an isoschizomer of SpeI (ACTAGT)? (A) EcoRI (GAATTC) (B) Xbal (TCTAGA) (C) BamHI (GGATCC) (D) SalI (GTCGAC) (E) Eagl (CGGCCG).

11. Which scientists proved that the genetic material is DNA, instead of protcins? (A) K. B. Mullis(B) J. D. Watson and H. C. Crick (C) A. D. Hershey and M. Chase(D) S. B. Prusiner (E) G. N. Ramachandran.

12. Tween-20 is a nonionic surfactant that denatures proteins by disrupting________? (A) hydrogen bonds (B) disulfide bridges (C) hydrophobic intera actions (D) salt bridges (E) covalent bonds.

13.How many stereoisomers for an aldopentose? (A) 2 (B) 4 (C) 6 (D) 8 (E) 16.

14. A phosphate is attached to a ribose through a________bond in DNA. (A) Ether (B) Ester (C) Amide (D) Aldehyde (E) Ketone.

15. Which of the following lipids is not a major comp onent of cellular memb ranes? (A) glycerophospholipids (B) sphingolipids (C) stearic acids (D) glycosphingolipids (E) glycoglycerolipid.

16. loops are found in (A) mRNA (B) rRNA (C) tRNA (D) hnRNA (E) snRNA.

17. Which of the following bond-pairs within a peptide backbone show free rotation around both bonds? (A) N-Ca and N-C (B) Ca-C and N-Ca (C) C=O and N-C (D) C=O and N-Ca (E) N-C and Ca-C.

18. Which scientist made a significant contribution to the discovery of prion? (A) K. B. Mullis (B) J. D. Watson and H. C. Crick (C) A. D. Hershey and M. Chase (D) S. B. Prusiner (E) F. Sanger.

19.Which of the following amino acids contains only one codon? (A) Ala (B) Lys (C) Tyr(D) Met (E) Arg.

20. In the universal codon table, how many of them are "nonsense codons"? (A) 0 (B) 1 (C) 2 (D) 3 (E) 4

21. The Western blotting method is normally used for detection of (A) proteins (B) DNA (C) RNA (D) lipids (E) carbohydrates.

22.Which of the following tautomeric forms is the major form of glucose in solution? (A) a-pyranose (B)β-pyranose (C) a-furanose (D) β-furanose (E) all of the above.

23. The ribosome-binding site has a sequence of______in E. coli? (A) AATT (B) AAGG (C) ATGC (D) TTCC (E) TTGG.

24._________ is poisonous, because it blocks electron fow from NADH to CoQ in the respiratory chain. (A) Rotenone (B) Antimycin A (C) Cyanide (D) Azide (E) CO

25. Which of the following compounds is a suicide inhibitor of aconitase? (A) citrate (B) fluoroacetate (C) isocitrate (D) acetyl-CoA (E) FADHz.

26. Which pair of enzymes listed below is unique to the glyoxylate cycle? (A) pyruvate dehydrogenase and fructose- bisphosphatase-1 (B) pyruvate ca rboxylase and 3-phosphoglycerate kinase (C) PEPCK and pyruvate carboxylase (D) citrate lyase and malate synthase (E) pyruvate carboxylase and phosphofructokinase-1.

27. The major monosaccharides in honey are (A) glucose and galactose (B) fructose and galactose (C) galactose and mannose (D) glucose and fructose (E) galactose and fructose.

28. Which of the following reactions is not located in mitochondria? (A) gluconeogenesis (B) tricarboxylic acid cycle (C) electron transport (D) β-oxidation (E) oxidative phosphorylation.

29.In a Lineweaver-Burk double reciprocal plot, the intercept of the y-axis equals_____. (A) K_M (B) -1/KM (C) (D) 1/ (E)