112年 - 112 國立成功大學_碩士班招生考試試題_藥理學研究所：生物化學#119951

科目：研究所、轉學考(插大)◆生物化學 | 年份：112年 | 選擇題數：30 | 申論題數：5

試卷資訊

所屬科目：研究所、轉學考(插大)◆生物化學

選擇題 (30)

1. Phenylketonuria is caused by the deficiency of which following enzyme ? (A) Aminotransferase； (B) Fumarylacetoacetase； (C) Maleylacetoacetate isomerase； (D) Phenylalanine bydroxylase.

2. Beriberi (腳氣病) is caused by the lacking of? (A) niacin； (B) riboflavin； (C) pyridoxine； (D) thiamin.

3. Administration of aspirin will inhibit the production of ? (A) Insulin； (B) Leukotriene； (C) Prostaglandin； (D) Testosterone.

4. Lovastatin is the drug for the treatment of familial hypercholesterolemia because it inhibits which of the following enzyme? (A) HMG-CoA synthase； (B) HMG-CoA lyase； (C) HMG-CoA reductase； (D) HMG-CoA isomerase.

5. Which of the following second messenger is associate with plasma membrane? (A) CAMP； (B) cGMP； (C) diacylglycerol； (D) IP3.

6. Which of the foLlowing proteins involved in signal transduction is not an integral membrane protein? (A) acetyl choline receptor； (B) estrogen receptor； (C) insulin receptor； (D) B-adrenergic receptor.

7. Which of the following ligand will activate a receptor tyrosine kinase? (A) epinephrine； (B) epidermal growth factor； (C) estrogen； (D) glucagon.

8. Which of the following description about insulin receptor is incorrect ? (A) insulin receptor is an oncoprotein； (B) insulin receptor is a dimeric transmembrane protein； (C) insulin receptor is a tyrosine kinase； (D) insulin receptor does not activate phosphatidyl inositol-3 kinase by itself.

9. Among 20 amino acids, how many of them contains -OH group in the side chain? (A) one； (B) two； (C) three； (D) four.

10. Which of the following methods separate proteins by their molecular weight? (A) gel filtration； (B) isoelectric precipitation； (C) ion exchange chromatography； (D) salting out.

11. Which of the following is not a secondary structure of a protein? (A) alpha-helix； (B) beta-pleated sheet； (C) collagen triple helix； (D) disulfide bridge

12. Which of the following represents the biding affinity between an enzyme and its substrate? (A) Vmax； (B) Kcat； (C) Km； (D) Kcat/Km.

複選題
13. As a noncompetitor, which of the following description is correct? (A) Km is not changed； (B) It has higher binding affinity； (C) Vmax is not changed； (D) The structures of noncompetitive inhibitor and ligand are similar.

14. Eukaryotic ribosomes contain 288 rRNA. What does 288 stand for ? (A) number of sugars； (B) sedimentation coefficient； (C) splicing coefficient； (D) splicing number.

15. Dehydrogenases are enzymes that: (A) Move hydrogens within the molecules； (B) Add hydrogens within the molecules； (C) Transfer hydrogens between substrates； (D) Transfer hydride ions to NAD⁺ (or NADP⁺) and release a proton.

16. Where are ketone bodies formed in the body ? (A) Cytosol of muscle cells； (B) Cytosol of brains cells； (C) Mitochondria of liver cells； (D) Mitochondria of muscle cells.

17. What is the final product of glucose metabolism in red blood cells? (A) acetyl-CoA； (B) CO₂； (C) lactate； (D) pyruvate.

18. Which of the following chemicals does not directly participate in the synthesis of ATP in cells ? (A) 1,3-Bisphosphoglycerate； (B) 3-Phosphoglycerate； (C) Phosphocreatine； (D) Phosphoenolpyruvate.

19. Enzymes involved in electron transport chain localize at ? (A) mitochondrial matrix； (B) cytosol； (C) mitochondria outer membrane； (D) mitochondrial inner membrane.

20. In aerobic electron transportation, the flow of electron terminates at ? (A) H₂O； (B) O₂； (C) coenzyme Q； (D) NAD⁺.

21. How many CO₂ will be released from one cycle of acetyl-CoA entering citric acid cycle (or TCA cycle) ? (A) 1； (B) 2； (C) 3； (D) 4.

22. In mitochondria, which of the followings is not caused by uncoupling protein ? (A) Mitochondrial proton gradient is lost； (B) ATP/ADP ratio is increased； (C) Heat is generated； (D) None.

23. Which of following reaction occurs in thodopsin stimulated by light ? (A) β-carotene turns into retinal； (B) retinal is reduced into retinol (C) cis-retinal is isomerized into all-trans-retinal； (D) all-trans-retinal is isomerized into cis-retinal

24. Which of the followings has similar chemical structure to hypoxanthine and is used to treat gout ? (A) allopurinol； (B) p-aminobenzoate； (C) 5-fluorouracil； (D) methotrexate.

25. Which of the following lipoproteins increased might contribute to the enhanced blood cholesterol? (A) VLDL； (B) LDL； (C) HDL； (D) All but C.

26. Which of the following enzymes is important for the synthesis of dTMP ? (A) dihydrouracil dehydrogenase； (B) dihydrofolate reductase； (C) xanthine oxidase； (D) 5'-nucleotidase.

27. Which of the following amino acids is the precursor of epinephrine synthesis? (A) glycine； (B) histidine； (C) tryptophan； (D) tyrosine.

28. NADPH generated from the following pathways is needed in the biosynthesis of fatty acid ? (A) glycolysis； (B) malic enzyme and pentose phosphate pathway； (C) oxidative phosphorylation； (D) TCA cycle.

29. In the pentose phosphate pathway, which of the followings is not an intermediate for DNA/RNA ribose formation from glucose ? (A) glucose-1-phosphate； (B) glucose-6-phosphate； (C) ribose-5-phosphate； (D) 6-phosphogluconate.

複選題
30. Atom N in purine and pyrimidine ring is provided from the metabolism of which following amino acids ? (A) Alanine； (B) Aspartate； (C) Glycine； (D) Glutamate.

申論題 (5)

1.試比較AstraZeneca/Oxford COVID-19 Vaccine與 ModernaBNT mRNA Vaccine的原理 (10%)

2.試寫出下列DNA template所轉錄(transcription)的RNA 序列(要標示5與3'end).(5%)

5'-ATG-ATG-CCT-AGG-ATC-CCT-AAG-3'

a) RT-PCR；

b) siRNA；

c) CRISPR gene editing.