所屬科目：研究所、轉學考(插大)◆生物化學
1. Common lesion found in DNA after exposure to ultraviolet light are not able to be repaired by (A) Nucleotide excision repair system (B) Recombinational repair system (C) DNA photolyase (D) Base excision repair system (E) Alkyltransferase
2.Which of the following types of bonds or interaction are likely to be involved in stabilizing the three-dimensional folding of most proteins (A) Electrostatic bonds (B) Phosphodiester bonds (C) Ester bonds (D) Peptide bonds (E) None
3.In the lactose operon model, the repressor protein binds to (A) An enhancer (B) An operator (C) A promoter (D) A TATA box (E) A start codon
4."Leucine zippers" are important in cellular regulation because they are (A) Structures with high redox potential (B) A unique DNA sequences for protein binding (C) A domain in many growth factor receptors (D) A motif in many DNA-binding proteins (E) At the catalytic site of many kinases
5.Which of the following is most likely to lead to a loss of gene function? (A) A change from a TGA codon to a TAA codon in the coding region (B) A change from C to T in an enhancer region (C) A change from A to G in the open reading frame (D) A frameshift mutation in promoter region (E) A single nucleotide polymorphism in coding region
6. Which of the following columns are used in purification of eukaryotic mRNA? (A) Ni-NTA-column (B) Oligo-dT column (C) Oligo-dG column (D) GST column (E) IgG column
7.Which of the following enzyme abnormalities result in hyperuricermia and gout? (A) Нурохanthine-guanine phosphoribosyltransferase (B) PRPP amidotransferase (C) PRPP synthetase (D) All above (E)None
8. Which of the following protein responds to damage DNA and induces activation of SOS regulon? (A) Lex A9. (B) Rec A (C) Sul A (D) Uvr A (E) Dna A
9.The peptide bond formation between two amino acids is catalyzed by (A) The large subunit of a ribosome (B) The small subunit of a ribosome (C) Aminoacyl tRNA synthetase (D) EF-Tu (E) 16S rRNA
10. Which of the following descriptions about eukaryotic mRNA is NOT TRUE? (A) Synthesize in the nucleus (B) Has 7-methylguanosine at the 5' end (C) Has the polyadenylation at 3' end (D) Contain introns (E) None
11. An enzyme is required for polymerase chain reaction(A) Telomerase (B) RNA polymerase (C) DNA polymerase (D) Helicase (E) RNase H
12. An enzyme is required for degradation of RNA(A) Telomerase (B) RNA polymerase (C) DNA polymerase (D) Helicase (E) RNase H
13. An enzyme is required for separation of two strands of DNA(A) Telomerase (B) RNA polymerase (C) DNA polymerase (D) Helicase (E) RNase H
14. An enzyme is required for replication of the end of eukaryotic chromosomes(A) Telomerase (B) RNA polymerase (C) DNA polymerase (D) Helicase (E) RNase H
15. A glyoxylate cycle enzyme(A) Arginase (B) Glucose-6-phosphate dehydrogenase (C) B-ketothiolase (D) Rubisco (E) Citrate synthease
16. A Krebs-Henseleit urea cycle enzyme(A) Arginase (B) Glucose-6-phosphate dehydrogenase (C) B-ketothiolase (D) Rubisco (E) Citrate synthease
17. An enzyme of the pentose phosphate pathway(A) Arginase (B) Glucose-6-phosphate dehydrogenase (C) B-ketothiolase (D) Rubisco (E) Citrate synthease
18. A Calvin cycle enzyme(A) Arginase (B) Glucose-6-phosphate dehydrogenase (C) B-ketothiolase (D) Rubisco (E) Citrate synthease
19. The size of human polymerase ẞ gene is (A) 6.62 KЬ (B) 4.32 Kb (C) 2.30 Kb (D) 1.15 Kb (E) 10.94 kb
20. Which of the following descriptions is TRUE? (A) Two HindIII cutting sites in the recombinant (B) Two EcoRI cutting sites in the recombinant (C) Two Notl cutting sites in recombinant (D) One HindIII cutting sites in the receombinant (E) One Notl cutting sites in the vector
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