複選題
36. For the DNA replication in E. coli, which of the following is correct?
(A) The leading strand is initially synthesized as Okazaki fragments
(B) It has the DNA strand synthesized in a 5'-3' direction
(C) DNA polymerase I adds nucleotides to the primer strand
(D) The leading strand is synthesized continuously
(E) It synthesizes each strand in the opposite direction (one 5'->3', one 3'->5'), so they can be synthesized at once.

複選題32. The ability of DNA to denature is important for which process? (A) DNA synthesis (B) nucleic acid hybridization experiments (C) RNA synthesis (D) PCR reaction (E) DNA separation by agarose gel electrophoresis

33. Which of the following is required for both DNA replication and RNA transcription? (A) DNA (B) primers (C) RNA polymerase (D) phosphodiesterase (E) topoisomerase

34. Which of the following mobile elements is a retrotransposon? (A) yeast Ty element (B) bacterial IS sequence (C) Drosophila P element (D) maize activator (Ac) element (E) RNA genome of influenza H7N9 virus

複選題35. Which of the following is correct for the eukaryotic chromosomes? (A) The ends of eukaryotic chromosomes are called telomeres, and contain special repeat sequences, called telomeric sequences. (B) Telomere terminal transferase containing the reverse transcriptional activity is important to maintain the chromosomal ends in eukaryotes. (C) The centromeric sequences are important for proper chromosome segregation during replication. (D) Kinetochores assemble at the centrosome. (E) Telomerase activity declines with age and this could explain why cells lose their ability to divide after many replications.

37. A mutation that changes the recognition sequence for the restriction enzyme EcoRI from GAATTC to GATTTC is an example of a (A) restriction fragment length polymorphism (RFLP). (B) single nucleotide polymorphism (SNP). (C) simple sequence repeat (SSR). (D) genetic heterogeneity (E) none of the above

38. When a segment of DNA duplicates, causing two or more copies of a gene to be present in the chromosome, the most unlikely fate of the duplicate(s) would be (A) to lose function in subsequent mutation. (B) to improve function in subsequent mutation. (C) to gain a new function in subsequent mutation. (D) to become a pseudogene. (E) to become part of a gene family.

39. There are 45 different kinds tRNA (anticodons) available to serve as amino acid carriers, but there are 64 mRNA codons. Why aren't the tRNA anticodons and mRNA codons equal in number? (A) The reason is that the third base pair on the tRNA allows some flexibility (wobble); thus, some tRNA anticodons can recognize more than one mRNA codon. (B) The reason is that some tRNA anticodons can misread some of the mRNA codons, which creates a "wobble" in the tRNA anticodons that can be repaired by RNA repair enzymes. (C) The reason is that the third base pair on the mRNA codon allows some flexibility (wobble); thus, some tRNA anticodons can recognize more than one mRNA codon. (D) The reason is that the tRNA has the flexibility to choose which mRNA codons are necessary for building the polypeptide chain. (E) This broader recognition occurs because of the nonstandard pairing between bases in the wobble position corresponding to the 3' base in the mRNA and the complementary 5′ base in the tRNA anticodon.

40. Which one of the following statements comparing the human and mouse genomes is false? (A) Human and mouse have about the same number of genes. (B) The human genome shares 99% of its genes with the mouse. (C) A comparison of genomes confirms that mouse and humans shared a common ancestor more recently than humans and pufferfish. (D) Mitochondrial DNA is paternally inherited in both human and mouse. (E) Little rearrangement of genes has occurred in the two genomes.

1. 給水、排水及衛生設備圖之圖號為 (A)P (B)S (C)M (D)L。

2. 有關拌合水泥砂漿之敘述，下列何者錯誤？ (A)拌合時應乾拌均勻後才可加水濕拌 (B)拌合水之潔淨度，會影響水泥砂漿的凝結強度 (C)拌合後之水泥砂漿應立即使用，不宜久置 (D)拌合水之多寡與工作度無關。