複選題
77.下列敘述何者為非？
(A)硫酸稀釋時，應將試劑水緩慢加入硫酸中，避免噴濺
(B)使用高壓氣 體鋼瓶，只要存放定位避免陽光曝曬，沒有洩漏 的 危害
(C) 實 驗 中應 避 免飲食 ， 可 將食 物 暫 存 實 驗 室內 冰 箱，待 實 驗 完畢 後 再行食 用
(D) 實驗 室 內應穿 著 實 驗衣 及 包覆式 鞋 子 。

複選題78.水中總氮的計量會納入下列哪些化合物？(A)硝酸鉀(B)醋酸銨(C)氮氣(D)尿素。

複選題79.下列何種檢測結果須以儀器判讀完成？(A)氯鹽(B)氨氮(C)硫酸鹽(D)鹼度。

複選題80.關於採樣容器的敘述何者錯誤？(A)採集揮發性有機物應使用棕色玻璃瓶，且瓶蓋內應附有 鐵氟龍(PTFE)的襯裏(B)採集多氯聯苯項目時必須 使 用玻璃 容 器 盛裝 (C) 採集之 水 樣 若使 用 正 確 的 容器 ， 且添加 保 存 試劑 ， 水質即 不 會 產生 變 化 (D)採 集 農 藥項 目 時可以 使 用 塑膠 瓶 盛 裝 。

1. The chirality of an amino acid results from the fact that its α carbon: (A) has no net charge. (B)is a carboxylic acid. (C) is bonded to four different chemical groups. (D)is in the L absolute configuration in naturally occurring proteins. (E)is symmetric.

複選題2. All of the amino acids that are found in proteins, except for proline, contain a(n): (A) amino group. (B)carbonyl group. (C) carboxyl group. (D)ester group. (E)thiol group.

3. The uncommon amino acid selenocysteine has an R group with the structure -CH2-SeH (pKa ≈ 5). In an aqueous solution, pH = 7.0, selenocysteine would: (A) be a fully ionized zwitterion with no net charge. (B)be found in proteins as D-selenocysteine. (C) never be found in a protein. (D)be nonionic. (E)not be optically active.

4. The first step in two-dimensional gel electrophoresis generates a series of protein bands by isoelectric focusing. In a second step, a strip of this gel is turned 90 degrees, placed on another gel containing SDS, and an electric current is again applied. In this second step: (A) proteins with similar isoelectric points become further separated according to their molecular weights. (B)the individual bands become stained so that the isoelectric focus pattern can be visualized. (C) the individual bands become visualized by interacting with protein-specific antibodies in the second gel. (D)the individual bands undergo a second, more intense isoelectric focusing. (E)the proteins in the bands separate more completely because the second electric current is in the opposite polarity to the first current.

5. Which of the following describes the overall three-dimensional folding of a polypeptide? (A) Primary structure (B)Secondary structure (C) Tertiary structure (D)Quaternary structure (E)None of the above

6. In an aqueous solution, protein conformation is determined by two major factors. One is the formation of the maximum number of hydrogen bonds. The other is the: (A) formation of the maximum number of hydrophilic interactions. (B)maximization of ionic interactions. (C) minimization of entropy by the formation of a water solvent shell around the protein. (D)placement of hydrophobic amino acid residues within the interior of the protein. (E)placement of polar amino acid residues around the exterior of the protein.

7. In the α helix the hydrogen bonds: (A) are roughly parallel to the axis of the helix. (B)are roughly perpendicular to the axis of the helix. (C) occur mainly between electronegative atoms of the R groups. (D)occur only between some of the amino acids of the helix. (E)occur only near the amino and carboxyl termini of the helix.

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