複選題
9. U-rich small nuclear RNAs (snRNAs) are important for base pairing with the pre-mRNA and are required for pre-mRNA splicing. They include
(A) U1
(B) U2
(C) U4
(D) U5
(E) U6

複選題5. Which mechanism(s) is/are often used in bacterial horizontal gene transfer? (A) Transformation (B) Transfection (C) Transduction (D) Conjugation (E) Transposon

複選題6. Which of the following biological mechanism(s) in the cell could be regulatory targets regarding gene expression? (A) Transcription (B) mRNA processing (C) mRNA export and localization (D) Translation (E) Protein modification

複選題7. Which RNA molecule(s) is/are transcribed by RNA polymerase II? (A) tRNA (B) rRNA (C) snRNA (D) mRNA (E) microRNA

複選題8. Which organism listed below would need spliceosome-based pre-mRNA splicing to remove introns? (A) Homo sapiens (B) Drosophila melanogaster (C) Caenorhabditis elegans (D) Saccharomyces cerevisiae (E) Escherichia coli

10. Which following description(s) is/are CORRECT about ribozyme? (A) A catalyst that uses RNA as substrate (B) An enzyme that synthesizes RNA as a part of the transcription process (C) An enzyme that catalyzes the association between the large and small ribosomal subunits (D) An RNA with catalytic activity (E) An important component of RNA transport from the nucleus to the cytoplasm

11.RNA interference (RNAi) is an evolutionarily conserved mechanism to degrade RNA molecules in a sequence-specific manner. This mechanism does NOT exist in (A) Homo sapiens (B) Drosophila melanogaster (C) Caenorhabditis elegans (D) Saccharomyces cerevisiae (E) Escherichia coli

複選題12.MicroRNAs are small non-coding regulatory RNAs that down regulate gene expression by the mechanisms including (A) Repress translation of target mRNAs (B) Trigger cleavage of target mRNAs (C) Trigger decapping of target mRNAs (D) Enhance polyadenylation of target mRNAS (E) Trigger the nonsense-mediated decay (NMD) pathway

13.During the first stage of translation, the small and large ribosomal subunits assemble around an mRNA that has an aminoacylated initiator tRNA correctly positioned at the start codon. The process is mediated by a special set of proteins known as translation initiation (IFs). In eukaryotes, the translation initiation complex contains the 40S subunit, the Met-tRNA and IFs NOT including (A) eIF1 (B) eIF2 (C) eIF3 (D) eIF4 (E) eIF6

複選題14. Which of the following techniques can be used to measure the amount of a specific mRNA in a sample? (A) Real-Time quantitative reverse transcription PCR (B) Primer extension analysis (C) Northern blot analysis (D) Southern blot analysis (E) Western blot analysis

複選題15. Which enzyme(s) is/are NOT required for setting up a Real-Time quantitative reverse transcription PCR experiment? (A) E. coli DNA polymerase I (B) T4 DNA ligase (C) Taq DNA polymerase (D) Reverse transcriptase (E) T7 RNA polymerase