10. If you want to clone a DNA fragment into vector shown below, what are required to select clones with insert DNA? (2 points)
（pUC19 plasmid diagram: lacZa, Polylinker, amp, ori 各功能區段）
A. Ampicillin
B. Lac operon
C. IPTG
D. Amphotericin B
E. X-gal.
F. Amphetamine
(A)AB
(B)CEF
(C)ACE
(D)BE

(E)CDE

1. Which one is the component of linker histone? (2 points)(A)H1(B)H2A(C)H2B(D)H3(E)H4

2. If a DNA strand contains the sequence 5'-ATTCCGGATCGA-3', which of the following is the sequence of the complementary DNA strand? (2 points)(A)5'-TAAGGCCTAGCT-3'(B)5'-ATTCCGGATCGA-3'(C)3'-TAACCGGTACGT-5'(D)5'-TCGATCCGGAAT-3'

3. During the denaturation of DNA, which of the following happens? (2 points)(A)Phosphodiester bonds break within the sugar-phosphate backbone.(B)Hydrogen bonds within the sugar-phosphate backbone break.(C)Hydrogen bonds between complementary bases break.(D)Phosphodiester bonds between complementary bases break.

4. Hemophilia (type A) is an X-linked recessive disease. Mary and her husband are both unaffected; however, their first son has hemophilia. What is the possibility of being affected by hemophilia for their next children? (2 points)(A)50%(B)25%(C)6.25%(D)12.5%

5. Three alleles of a polymorphic locus have been found in a particular gene of a diploid organism. If all homozygous are lethal, how many different genotypes will be observed? (2 points)(A)12(B)3(C)9(D)6

6. A previously unknown cell type is discovered in the central nervous system of a laboratory strain of mice. In cell-culture experiments, researchers find that the cell type can self-renew, but can only differentiate into one cell type. Which of the following best characterizes this cell type? (2 points)(A)Unipotent embryonic stem cell(B)Unipotent somatic stem cell(C)Multipotent somatic stem cell(D)Multipotent embryonic stem cell

7. Which of the following principal bases of nucleic acid are pyrimidines? (2 points)A. AdenineB. UracilC. ThymineD. GuanineE. Cytosine(A)AD(B)BCE(C)ABC(D)DE(E)CE

8. Synaptic signaling between adjacent neurons is like hormone signaling in which of the following ways? (2 points)(A)It sends its signal molecules through the blood.(B)It sends its signal molecules quite a distance.(C)It requires calcium ions.(D)It requires the binding of a signaling molecule to a receptor.(E)It persists over a long period.

9. Which of the following controls the development of an adult structure? (2 points)(A)homeotic gene(B)structural gene(C)regulator gene(D)proto-oncogene

11. There are four polypeptides with the following primary sequences (from the N-terminus to the C-terminus): (2.5 points)(1) MSDSQNPWGGGGGGGGGGAAAAAAAAKYCHT(2) MSDSKKQRGGGGGGGGGGAAAAAAAAAIKRKT(3) MSAMSLNVGGGGGGGGGGAAAAAAAAALIPDH(4) MTSMCDDEGGGGGGGGGGAAAAAAAAEDDCYWhich polypeptide is most suitable for purification by cation exchange chromatography?(A)Polypeptide (1)(B)Polypeptide (2)(C)Polypeptide (3)(D)Polypeptide (4)

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