試題詳解

13. 在進行 PCR 反應時，請問下列何組順向引子 (forward primer)與反向引子 (reverse primer) 可用來擴增以下 DNA 片段：
5'-AGCCCGGAGAGAGGGGGAAGAAGAGAATCCCAAGGGAGCGGGAATAGCCGTGCGGAAGAGAAGAC GATTAGCCTAGGTAGAAGCCTCATGGCTGACCATTTGATTCCGAAAAGCTGGCAAAACATTCACGAGATA GACATAGTGAGGACCCGGACGATGTCCCAAGCGGCGCGCTGATTGGCCAGGCCGGGCAGTGCCGAAGCA GCAAATATCGTGAGTCTCCTGCTTTGCCCGGTGTATGAAACCGGTAAGGGTTGCTGGGGAGCTGGTCAGC GGCGCAAGCCGGGAGGGCGACTGAGC-3'  
(A)順向引子：5'-AGCCCGGAGAGAGGGGGA-3'；反向引子：5'-CCGGGAGGGCGACTGAGC-3'
(B)順向引子：5'-AGCCCGGAGAGAGGGGGA-3'；反向引子：5'-GCTCAGTCGCCCTCCCGG-3'
(C)順向引子：5'-AGCCCGGAGAGAGGGGGA-3'；反向引子：5'-CGAGTCAGCGGGAGGGCC-3'
(D)順向引子：5'-CCGGGAGGGCGACTGAGC-3'；反向引子：5'-AGGGGGAGAGAGGCCCGA-3'
(E)順向引子：5'-GCTCAGTCGCCCTCCCGG-3'；反向引子：5'-AGCCCGGAGAGAGGGGGA-3'