27. Please choose the correct answer from the following descriptions: 1. Number of hydrogen bonds per water molecule in ice is higher than that in water. 2. 0.1 g glycogen in 100 mL water results in lower osmolarity than 0.1 g glucose in 100 mL water. 3. Every turn of α-helix is 5.4 Å in length. 4. The strength of α-keratin depends on its Cys content. 5. Hydrogen bond in α-helix formed between residue i and i+3. (A) 1 and 3 are incorrect. (B) 2 and 5 are incorrect. (C) Only 1, 2 and 4 are correct. (D) Only 2 is incorrect. (E) Only 5 is incorrect.

27. Please choose the correct answer from the following descriptions:

1. Number of hydrogen bonds per water molecule in ice is higher than that in water.

2. 0.1 g glycogen in 100 mL water results in lower osmolarity than 0.1 g glucose in 100 mL water.

3. Every turn of α-helix is 5.4 Å in length.

4. The strength of α-keratin depends on its Cys content.

5. Hydrogen bond in α-helix formed between residue i and i+3.
(A) 1 and 3 are incorrect.
(B) 2 and 5 are incorrect.
(C) Only 1, 2 and 4 are correct.
(D) Only 2 is incorrect.
(E) Only 5 is incorrect.

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1. 題目解析這道考題要求我們判斷五個...

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30. A protein mixture contains protein A, B, C. Protein A: homodimer Mr = 180,000, pI 5； Protein B: Mr = 12,000, pI 7； Protein C: M____r = 150,000, pI 9 Joseph says: When I used SDS-PAGE to separate these three proteins. The sequence of the protein bands shown in gel from top to bottom is A-C-B. Steve says: When I used SDS-PAGE to separate these three proteins. The sequence of the protein bands shown in gel from top to bottom is B-A-C Jacky says: When I dissolved the protein mixture in 20 mM phosphate buffer (pH 7) and loaded it to a cation exchange column equilibrated with the same buffer. Only protein C could bind to the column matrix Simon says: When I dissolved the protein mixture in 20 mM phosphate buffer (pH 8) and loaded it to an anion exchange column equilibrated with the same buffer. Only protein C could bind to the column matrix Clarence says: When I dissolved the protein mixture in 150 mM phosphate buffer (pH 7) and used a gel filtration column to separate them, the elution sequence is B→C→A. Rita says: When I dissolved the protein mixture in 150 mM phosphate buffer (pH 7) and used a gel filtration column to separate them, the elution sequence is A→C→B. Lily says: When I dissolved the protein mixture in 150 mM phosphate buffer (pH 7) and used a gel filtration column to separate them, the elution sequence is C→A→B. Who are correct? (A) Joseph, Simon, and Clarence. (D) Simon and Clarence. (B) Rita and Jacky. (E) Joseph, Jacky, and Rita. (C) Steve, Jacky, and Lily.

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