30. A protein mixture contains protein A, B, C.
Protein A: homodimer Mr = 180,000, pI 5; Protein B: Mr = 12,000, pI 7; Protein C: M____r = 150,000, pI 9
Joseph says: When I used SDS-PAGE to separate these three proteins. The sequence of the protein bands shown in gel from top to bottom is A-C-B.
Steve says: When I used SDS-PAGE to separate these three proteins. The sequence of the protein bands shown in gel from top to bottom is B-A-C Jacky says: When I dissolved the protein mixture in 20 mM phosphate buffer (pH 7) and loaded it to a cation exchange column equilibrated with the same buffer. Only protein C could bind to the column matrix
Simon says: When I dissolved the protein mixture in 20 mM phosphate buffer (pH 8) and loaded it to an anion exchange column equilibrated with the same buffer. Only protein C could bind to the column matrix
Clarence says: When I dissolved the protein mixture in 150 mM phosphate buffer (pH 7) and used a gel filtration column to separate them, the elution sequence is B→C→A. Rita says: When I dissolved the protein mixture in 150 mM phosphate buffer (pH 7) and used a gel filtration column to separate them, the elution sequence is A→C→B.
Lily says: When I dissolved the protein mixture in 150 mM phosphate buffer (pH 7) and used a gel filtration column to separate them, the elution sequence is C→A→B.

Who are correct?
(A) Joseph, Simon, and Clarence.
(D) Simon and Clarence.
(B) Rita and Jacky.
(E) Joseph, Jacky, and Rita.
(C) Steve, Jacky, and Lily.

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