31. Transamination requires which of the cofactors
(A) Mg,
(B) B12,
(C) Fe,
(D) PLP,
(E) NAD

32. S-adenosylmethionine is involved in the transfer of (A) CH3, (B) CH2OH, (C) CHO, (D) COOH, (E) NH2

33. Which of the following methods was NOT invented to increase the throughput of DNA deep sequencing? (A) Pyrosequencing, (B) Sequencing by ligation, (C) Sequencing by synthesis, (D) Sanger sequencing, (E) semiconductor sequencing

34. The corresponding alpha-keto acid after aspartate deamination is (A) fumarate, (B) oxaloacetate, (C) pyruvate, (D) alpha-ketoglutarate, (E) citrate

35. One turn of the citric acid cycle produces (A) 2 NADH, 2 FADH2, 2 ATP. (B) 3 NADH, 1 FADH2, 1 ATP. (C) 1 NADH, 3 FADH2, 2 ATP. (D) 3 NADH, 2 FADH2, 1 ATP. (E) 2 NADH, 2 FADH2, 1 ATP.

36. The anaerobic conversion of 1 mol of glucose to 2 mol of lactate by fermentation is accompanied by a net gain of: (A) 2 mol of ATP+ 1 mol of NADH (B) 2 mol of ATP + 2 mol of NADH (C) 2 mol of NADH (D) 1 mol of NADH (E) 2 mol of ATP

37. Which of the following enzyme does not take part in the TCA cycle? (A) Citrate synthase (B) Malate dehydrogenase (C) Aconitase (D) Pyruvate dehydrogenase (E) Succinate dehydrogenase

38. Which of the following statements about the reactions of glycolysis is correct? (A) glucose-6-phosphate is split into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. (B) fructose 1,6-bisphosphate is split into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. (C) fructose-6-phosphate is split into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. (D) glucose-6-phosphate is isomerized to fructose 1,6-bisphosphate. (E) glucose 2,6-bisphosphate is split into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate.

39. All are true for translation in eukaryotic cells EXCEPT: (A) transcription and translation are spatially separated. (B) mRNAs are monocistronic. (C) transcription occurs in the nucleus. (D) primary transcripts undergo processing to mature mRNAs. (E) all are true.

40. Most eukaryotic RNA consists of coding regions, called _______, and noncoding regions, called _______. (A) introns； exons (B) exons； introns (C) spliceons； codons (D) codons; spliceons (E) introns; codons

41. The adapter molecule that interacts specifically with both nucleic acids and amino acids is: (A) rRNA. (B) tRNA. (C) mRNA. (D) ssRNA. (E) all are true.

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