32. Monoclonal antibodies   CANNOT   be used for
(A) Plasmid cloning
(B) ChIP assay
(C) ELISA
(D) Western blotting

33. Which drug interferes with peptidoglycan synthesis in prokaryotes? (A) erythromycin (B) penicillin (C) kanamycin (D) chloramphenicol

34. Which of the following descriptions is   NOT   true for plasmids? (A) Plasmid are found in bacteria (B) Plasmids are usually circular (C) Plasmids are found in eukaryotes (D) Plasmid are found in archaea

35. Which of the following techniques is used to study the interaction between DNA and protein? (A) DNA footprinting (B) Electrophoretic mobility shift assay (C) Chromatin immunoprecipitation assay (D) All of the above

36. Which of followings is the template of reverse transcriptase? (A) single-strand DNA. (B) single-strand RNA. (C) double-strand DNA. (D) double-strand RNA.

37. What is the role of Kozak sequence? (A) Initiation of RNA transcription (B) Ribosomal binding site (C) Initiation of protein translation (D) Transcription factor binding site

38. Protein A-conjugated agarose is commonly used to perform immune precipitation (IP) experiment because it can bind to antibody molecules through (A) Fab region (B) Fc region (C) hinge region (D) None of the above.

39. Which of the followings is involved in the processes of protein folding? (A) Hydrogen bond formation (B) Disulfide bond formation (C) Binding of heat shock proteins (D) All of the above

40. Which statement is correct for the chromatic remodeling process? (A) It is a spontaneous process that does not require the energy. (B) It confers the displacement and reorganization of nucleosomes that occurs in coordination with the activation of gene transcription. (C) It often coordinates with the initiation of translation. (D) The chromatin remodeling complex itself has the specificity that determines the particular target for transcription.

1. 請問大眾捷運系統主管機關的說明(I)在中央為交通部；(II)在直轄市為直轄市政府； (III)在縣 (市)為縣(市)政府，以下選項何者正確？ (A) II、III (B) I、II (C) I、III (D) I、II、III。

2. 下列何者為捷運系統之資本成本項目？ (A)運輸成本 (B)營運成本 (C)工程意外事故成本(D)初期投資成本。

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