42. Shuttle vectors have the property that they:
(A) contain promoters for the expression of the gene.
(B) have origins of replication for two different cell types, usually bacteria and yeast.
(C) are capable of incorporating very large DNA fragments.
(D) contain more than one antibiotic resistant gene.
(E) None of the above.

58. As shown in Figure (b) below, a point-like ball with initial velocity v0 is inclined at an angle of Ψ on the inclined plane and the inclined plane itself is inclined at an angle of Φ. We know that the distance from the point of launch to the point at which the ball strikes the incline, measured along the incline without taking into account air resistance, is  Therefore, as shown in Figure (b), what value of θ0 will maximise R when thrown from a projector with a height h = 2.22 m above the ground and an initial velocity v0 = 14.0 m/s? (Hint: Solve a quadratic equation of sing and, then, take an approximation: sin6° ≈ 1/10.) (A) smaller than 40° (B) about 42° (C) 45°, exactly (D) about 47° (E) larger than 50°

59. Which of the following statements about Maxwell's equations is false? (A) Gauss's law for magnetism implies that there are no magnetic monopoles. (B) Gauss's law for the electric field shows us that an electric field points away from positive charges and towards negative charges. (C) Faraday's law states that the rate of change of the magnetic flux through the enclosed surface of a circuit induces a current. (D) Maxwell's modification to Ampère's law gives that a time-varying electric field results in a circular magnetic field. (E) None of the above.

60. Which of the following values is closest to the inductance required to store 1.0 kWh of energy in a coil carrying a 200-A current? (A) 0.1 Η (B) 1 H (C) 10 H (D) 40 H (E) 200 Η

41. Glucose most commonly forms which of the following structures? (A) a pyranose using the hydroxyl group on carbon 4 (B) a pyranose using the hydroxyl group on carbon 5 (C) a pyranose using the hydroxyl group on carbon 6 (D) a furanose using the hydroxyl group on carbon 3 (E) a furanose using the hydroxyl group on carbon 4

43. E'° of the NAD+/NADH half reaction is -0.32 V. The E'° of the oxaloacetate/malate half reaction is -0.175 V. When the concentrations of NAD+, NADH, oxaloacetate, and malate are all 10⁻5 M, the "spontaneous" reaction is: (A) malate + NAD⁺ → oxaloacetate + NADH + H⁺. (B) malate + NADH + H⁺ → oxaloacetate + NAD⁺. (C) NAD⁺ + NADH + H⁺ → malate + oxaloacetate. (D) NAD⁺ + oxaloacetate → NADH + H⁺ + malate. (E) oxaloacetate + NADH + H⁺ → malate + NAD⁺.

44. Fructose in the diet or fructose from sucrose in the diet can be a source of calories for fat synthesis in the liver because: (A) ketoses are fattening. (B) fructose enters glycolysis after the primary regulation point, PFK-1. (C) fructose provides a net of four ATP from glycolysis. (D) fructose enters the branch of glycolysis that forms fat. (E) glycerol (the "backbone" of triacylglycerols) comes specifically from fructose.

45. Many of the enzymes of the Calvin cycle are also involved in __________; however, the glyceraldehyde-3-phosphate dehydrogenase of the Calvin cycle is specific for__________. (A) glycolysis; NADPH (B) citric acid cycle; NADH (D) citric acid cycle; NADPH (E) None of the above. (C) glycolysis; FADH₂

46. A mutase catalyzes which of the reactions, and belongs to which class of enzymes? (A) 2-phosphoglyceratephosphoenolpyruvate + H₂O; lyase (B) pyruvate + NADH + H⁺ lactate + NAD⁺; oxidoreductase (C) 3-phosphoglycerate 2-phosphoglycerate; isomerase (D) dihydroxyacetone phosphateglyceraldehyde-3-phosphate; isomerase (E) glucose-6-phosphatefructose-6-phosphate; isomerase

47. Which of the following statements about the reactions involved in the synthesis of deoxyribonucleotides is false? (A) Deoxyribonucleotides are derived from the corresponding ribonucleotides by direct reduction at the 2'-carbon atom of the D-ribose to form the 2'-deoxy derivative. (B) Formation of deoxyribonucleotides is catalyzed by ribonucleotide reductase, in which its substrates are ribonucleoside diphosphates. (C) Ribonucleotide reductase is notable in that its reaction involves free radicals in biochemical transformations. (D) Nucleoside diphosphate kinase catalyzes the reaction: dTDP + ATP → dTTP + ADP. (E) The immediate precursor of thymidylate (dTMP) is dUMP. The conversion of dUMP to dTMP is a reaction of deamination.

48. Consider a liver cell carrying out the oxidation of glucose under aerobic conditions. Suppose that we added a very potent and specific inhibitor of the mitochondrial ATP synthase, completely inhibiting this enzyme. Which of the following descriptions is(are) correct?1. The rate of oxygen consumption will increase.2. ATP production in the cell will quickly drop to zero.3. The citric acid cycle will speed up to compensate.4. Mitochondrial ATP synthesis will cease, but to compensate, cells will accelerate the production of ATP by glycolysis, preventing ATP levels from dropping to zero. 5. The cell will switch to fatty acid oxidation as an alternative to glucose oxidation. (A) 1 and 5 are correct. (D) Only 4 is correct. (B) 3 and 4 are correct. (E) Only 5 is correct. (C) Only 2 is correct.